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Biochemistry

6th Edition
Reginald H. Garrett + 1 other
ISBN: 9781305577206

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BuyFindarrow_forward

Biochemistry

6th Edition
Reginald H. Garrett + 1 other
ISBN: 9781305577206
Textbook Problem

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Protein Structure Evaluation Based on Gel Filtration Data A new protein of unknown structure has been purified. Gel filtration chromatography reveals that the native protein has a molecular weight of 240,000. Chromatography in the presence of 6 M guanidine hydrochloride yields a single peak corresponding to a protein of Mr 60.000. Chromatography in the presence of 6 M guanidine hydrochloride and 10mM β -mercaptoethanol yields peaks for proteins of Mr 34,000 and 26,000. Explain what can be determined about the structure of this protein from these data.

Interpretation Introduction

Interpretation:

What can be determined about the structure of this protein from the given data should be explained.

Concept Introduction:

Gel filtration is a separation technique of proteins, peptides and oligonucleotides based on the size. The sample to be separated is passed through a vertical column packed with fine porous beads. When the sample is draining through the gel bed, the molecules are passively distributed and enter into the pores. Larger molecules will elute first as they cannot enter into the pores. Smaller ones enter into the pores and stay sometimes there and elute slowly. Then the elution time is compared with a set of molecules with known molar masses.

Explanation

At higher concentrations of guanidine chloride denature the protein and make it lose the ordered structure and form randomly coiled structure. In this case the native protein has a molecular weight of 240 kD . When it denatures in the presence of 6 M Guanidine chloride, gel filtration yields a single peak corresponding to 60 kD . Therefore, we can conclude that this protein is a tetramer which has four subunits each with 60 kD

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