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Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Protein Structure Evaluation Based on Gel Filtration Data A new protein of unknown structure has been purified. Gel filtration chromatography reveals that the native protein has a molecular weight of 240,000. Chromatography in the presence of 6 M guanidine hydrochloride yields a single peak corresponding to a protein of M r 60.000. Chromatography in the presence of 6 M guanidine hydrochloride and 10mM β -mercaptoethanol yields peaks for proteins of M r 34,000 and 26,000. Explain what can be determined about the structure of this protein from these data.

BuyFind

Biochemistry

6th Edition
Reginald H. Garrett + 1 other
Publisher: Cengage Learning
ISBN: 9781305577206
BuyFind

Biochemistry

6th Edition
Reginald H. Garrett + 1 other
Publisher: Cengage Learning
ISBN: 9781305577206

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Chapter
Section
Chapter 6, Problem 7P
Textbook Problem

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Protein Structure Evaluation Based on Gel Filtration Data A new protein of unknown structure has been purified. Gel filtration chromatography reveals that the native protein has a molecular weight of 240,000. Chromatography in the presence of 6 M guanidine hydrochloride yields a single peak corresponding to a protein of Mr 60.000. Chromatography in the presence of 6 M guanidine hydrochloride and 10mM β -mercaptoethanol yields peaks for proteins of Mr 34,000 and 26,000. Explain what can be determined about the structure of this protein from these data.

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