Biology: The Dynamic Science (Mind...

4th Edition
Peter J. Russell + 2 others
Publisher: Cengage Learning
ISBN: 9781305389892

Biology: The Dynamic Science (Mind...

4th Edition
Peter J. Russell + 2 others
Publisher: Cengage Learning
ISBN: 9781305389892


Chapter 7, Problem 13TYK
Textbook Problem

There are several ways to measure cellular respiration experimentally. For example, CO2 and O2 gas sensors measure changes over time in the concentration of carbon dioxide or oxygen, respectively. Design two experiments to test the effects of changing two different variables or conditions (one per experiment) on the respiration of research organism of your choice.

Expert Solution
Summary Introduction

To analyze:

Designing of two experiments to test the effect of two different variables or conditions on the respiration process of pear cells (Pyrus cummunis).


The process of respiration plays a critical role in overall metabolism of a plant or an animal. Thus it often used as a general measure of metabolic rate. To preserve the fruits for a long time, the process of respiration should be controlled, to slow down the metabolic rates. The oxygen and carbon dioxide concentration along with the temperature influence the rate of respiration thus can be considered as the important variables which directly affects the process of respiration.

Explanation of Solution

Temperature is one of the most important factor which affects the respiration rate of the plants, and thus is the reason for maintaining the controlled temperature condition for the long term storage of the fruits, especially for the climacteric fruits. The atmospheric CO2 concentration also affects the rate of respiration. To determine the effects of CO2 on the respiration in isolated pear protoplast, aqueous solutions of sodium bicarbonate can prepared in molar concentrations which is calculated by the Henderson–Hasselbalch equation to produce a pH = 7...

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