Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 7.6, Problem 1TQ
Summary Introduction
To review:
The movement of a relaxed and supercoiled plasmid in the agarose gel.
Introduction:
A gel-like polysaccharide substance which is extracted from the red seaweed is known as agarose. A method of gel electrophoresis which is used to separate the mixed population of the macromolecules such as DNA (deoxyribonucleic acid) or proteins is known as agarose gel electrophoresis.
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Using the figure below, what is molecule "A"
(type a 1, 2 or 3 in the blank)
nuclease
ligase
DNA polymerase
What is the function of molecule "A"?
to separate the double helix into two
to piece together the Okazaki segments
to copy the new DNA strand to the old strand by complementary base pairing
Using the figure below, what is molecule "G"
(type a 1, 2 or 3 in the blank)
nuclease
ligase
DNA polymerase
What is the function of molecule "G"?
to separate the double helix into two
to piece
Using the figure below, what is molecule "A"
(type a 1, 2 or 3 in the blank)
nuclease
ligase
DNA polymerase
What is the function of molecule "A"?
to separate the double helix into two
to piece together the Okazaki segments
to copy the new DNA strand to the old strand by complementary base pairing
Using the figure below, what is molecule "G"
(type a 1, 2 or 3 in the blank)
nuclease
ligase
DNA polymerase
What is the function of molecule "G"?
to separate the double helix into two
to piece together the Okazaki segments
to copy the new DNA strand to the old strand by complementary base pairing
Which of the following statements best describes why one of the daughter strands is synthesized in pieces?
the enzymes that synthesize DNA are slower that the enzymes that unwind the double helix and this produces 'lagging time'
the enzymes that synthesize DNA can only do so in a 5' --->3' direction
this figure illustrates a eukaryotic cell since prokaryotic cells do not synthesize DNA…
EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents premature cell lysis due to osmosis in cells with weakened cell walls.
You have made a batch of resuspension solution to isolate plasmid DNA, but you forgot to add EDTA. What do you think will happen when you try to perform a plasmid isolation procedure with this reagent? Why?
Chapter 7 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 7.2 - Prob. 1TQCh. 7.2 - Prob. 2TQCh. 7.2 - Prob. 3TQCh. 7.2 - Prob. 4TQCh. 7.3 - Prob. 1TQCh. 7.3 - Prob. 2TQCh. 7.3 - Prob. 3TQCh. 7.3 - Prob. 4TQCh. 7.3 - Prob. 5TQCh. 7.6 - Prob. 1TQ
Ch. 7.6 - Prob. 2TQCh. 7.6 - Prob. 3TQCh. 7 - Prob. 1RQCh. 7 - Prob. 2RQCh. 7 - Prob. 3RQCh. 7 - Prob. 4RQCh. 7 - Prob. 5RQCh. 7 - Prob. 6RQCh. 7 - Prob. 7RQCh. 7 - Prob. 8RQCh. 7 - Prob. 9RQCh. 7 - Prob. 10RQCh. 7 - Prob. 11RQCh. 7 - Prob. 12RQCh. 7 - Prob. 13RQCh. 7 - Prob. 14RQCh. 7 - Prob. 15RQCh. 7 - Prob. 1TQCh. 7 - Prob. 2TQCh. 7 - Prob. 3TQ
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- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityarrow_forwardA solution contains DNA polymerase and the Mg ²+ salts of dATP, dGTP, dCTP, and TTP. The following DNA molecules are added to aliquots of this solution. Which of them would lead to DNA synthesis? (a) A single-stranded closed circle containing 1000 nucleotide units. (b) A double-stranded closed circle containing 1000 nucleotide pairs. (c) A single-stranded closed circle of 1000 nucleotides base-paired to a linear strand of 500 nucleotides with a free 3' -OH terminus. (d) A double-stranded linear molecule of 1000 nucleotide pairs with a free 3’-OH group at each end.arrow_forwardHow many microliters of the pGLO plasmid do you need if you want 5 micrograms of DNA and the plasmid concentration is 100 nanograms/microliter?arrow_forward
- All of the following statements are correct EXCEPT a. DNA forms described by Watson and Crick have right handed helix except of Z-DNA form b. In case of Z-DNA form, the deoxyribose phosphate backbone forms a "Zigzag structure" c. Inverted DNA sequence repeat that happens within each individual strand of the DNA, called cruciform d. Plasmid carries genes that convey antibiotic resistance to the host bacteriumarrow_forwardHow would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7% agarose gel slab? For separating small fragments of DNA, is it better to use a 7% gel or a 0.1% gel? Why?arrow_forwardIf the following piece of the partially double stranded DNA: 5' ATCG 3' 3' TAGCGGCATCCG 5' and add DNA polymerase, dTTP,dGTP and dCTP, what will be the sequence of the nucleotides that will be added? A. 5' ATCGCCGTAGGC 3' B 5' GGCATCCG 3' C. 5' CCGT 3' D 5'CCGTAGGC 3' E 5'GGC 3'arrow_forward
- When a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid, how many bands can be visualized on an agarose gel? a)1 b)2 c)5 d)3arrow_forwardGiven the sequence of DNA strand 1, determine the correct sequence for DNA strand 2 based on nucleotide base-pairing rules. DNA strand 1: 5’ A T G A G A C T G A T T 3’ DNA strand 2:arrow_forwardWhen DNA replication was investigated by using heavy, N15 DNA to mark the original molecules, and light, N14 DNA to mark the newly synthesized molecules, one band was found in the middle of the centrifuge column after one round of replication, and two bands were found (middle and top of column) after 2 rounds of replication. Imagine that after 1 round of replication 2 bands were found, one at the bottom and one at the top of the centrifuge column. In that case, what model of DNA replication would have been supported? The dispersive model The conservative model The Franklin model The semi-conservative modelarrow_forward
- Which of the following types of DNA damage would be hardest to repair using the DNA repair pathways?A. Complete removal of three nucleotides in the middle of one strand.B. A covalent bond between a base on one strand and a base on the complementary strand.C. Incorporation of a sugar other than deoxyribose into one strand.D. Covalent attachment of a short polypeptide to a single base.E. A covalent bond between a base and a deoxyribose on the same strand. Please explain why it's Barrow_forwardSuppose a piece of DNA is cut into four fragments of varying sizes. Where would you expect to find the larger fragments on the gel (top, middle, or bottom)? Why? Suppose you had 1000 pieces of each of the four fragments, how many bands would appear on the gel?arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forward
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