Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
6th Edition
ISBN: 9780815344322
Author: Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher: W. W. Norton & Company
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Chapter 9, Problem 1P
Summary Introduction

To examine: Whether the statement “Because the DNA double helix is only 2 nm wide-well below the limit of resolution of the light microscope-it is impossible to see chromosomes in living cells without special stains” is true or false.

Introduction: The light microscope is a type of instrument that uses the visible light to observe the images of small objects. The limit of resolution of the light microscope is set by the wavelength of visible light that ranges from 0.4 μm to 0.7 μm.

Expert Solution & Answer
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Answer to Problem 1P

Correct answer: The statement is true.

Explanation of Solution

The objects that are 500nm wide are able to detect clearly under the light microscope. However, smaller than this size appears blur due to wave-like nature of light. The DNA double helix is only 2 nm wide. Thus, it is below the limit of resolution of the light microscope. Hence, it is not possible to see the chromosomes in living cells under the light microscope without special stain. Hence, the statement is true.

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