Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 9.3, Problem 1TQ
Summary Introduction
To review:
The effect of presence of mutants on the estimation of mutation rates.
Introduction:
Mutation is a process by which the DNA (deoxyribonucleic acid) is transformed or damaged in a way that there is an alteration in the genetic information carried by the gene. The agents that cause mutations are called mutagens. Mutation can be caused as a result of exposure to harmful chemicals or radiation. The mutation rate is defined as the number of mutations formed per cell division.
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In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets.
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Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand?
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Production of a recombinant protein by E. coli is proportional to cell growth. Ammonia is used as a source of N and glucose as a source of C, under aerobic conditions. The recombinant protein has a general formula CH1.55N0.31O0.25 and the cell CH1.77N0.4900.24. The yield of biomass from glucose is 0.48 gcel/gglic, and the yield of recombinant protein from glucose is about 20% of that for biomass.a) How much ammonia is needed to produce 50 g of cells producing the recombinant protein?b) What is the oxygen demand in this process?c) For the cultivation of a wild E. coli not producing the recombinant protein, how different would the ammonia and oxygen demand be if the biomass yield remained at 0.48 gcel/gglyc ?
Chapter 9 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 9.1 - Prob. 1TQCh. 9.1 - Prob. 2TQCh. 9.1 - Prob. 3TQCh. 9.1 - Prob. 4TQCh. 9.2 - Prob. 1TQCh. 9.3 - Prob. 1TQCh. 9.4 - Prob. 1TQCh. 9.4 - Prob. 2TQCh. 9.5 - Prob. 1TQCh. 9.6 - Prob. 1TQ
Ch. 9 - Prob. 1RQCh. 9 - Prob. 2RQCh. 9 - Prob. 3RQCh. 9 - Prob. 4RQCh. 9 - Prob. 5RQCh. 9 - Prob. 6RQCh. 9 - Prob. 7RQCh. 9 - Prob. 8RQCh. 9 - Prob. 9RQCh. 9 - Prob. 10RQCh. 9 - Prob. 11RQCh. 9 - Prob. 12RQCh. 9 - Prob. 13RQCh. 9 - Prob. 14RQCh. 9 - Prob. 15RQCh. 9 - Prob. 16RQCh. 9 - Prob. 1TQCh. 9 - Prob. 2TQCh. 9 - Prob. 3TQCh. 9 - Prob. 4TQCh. 9 - Prob. 5TQCh. 9 - Prob. 6TQ
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