Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 9.4, Problem 1CC
Summary Introduction
To determine:
The expected fractions of light DNA and half-heavy DNA if the Meselson and Stahl experiment is conducted for four rounds of
Introduction:
Meselson and Stahl were able to demonstrate semi-conservative replication in E. coli by using isotopes of nitrogen to label the DNA and determining the relationship of original and new DNA strands by density differences of the new DNA.
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What observation in the Meslson and Stahl experiment to decipher the DNA replication model distinguishes the semi-conservative from the dispersive mode of replication? Explain.
Why were Meselson and Stahl unable to distinguish between the dispersive and the semiconservative models following only one round of DNA replication?
Assuming DNA replicates semi-conservatively, which of these most closely approximates what you would see after one generation if you were to perform the Meselson-Stahl experiment?
one band
two bands
three bands
Chapter 9 Solutions
Principles of Biology
Ch. 9.1 - Look ahead to Figure 17.10. How does bacterial...Ch. 9.1 - Prob. 1TYKCh. 9.2 - Prob. 1CCCh. 9.2 - Prob. 2CCCh. 9.2 - Prob. 3CCCh. 9.2 - A nucleotide composed of deoxyribose, phosphate,...Ch. 9.2 - Prob. 2TYKCh. 9.2 - Prob. 3TYKCh. 9.3 - Prob. 1TYKCh. 9.3 - To determine the structure of DNA, Watson and...
Ch. 9.4 - Prob. 1CCCh. 9.4 - Prob. 1TYKCh. 9.4 - Prob. 2TYKCh. 9.5 - Prob. 1CCCh. 9.5 - Prob. 1TYKCh. 9.5 - Prob. 2TYKCh. 9.5 - Prob. 3TYKCh. 9.6 - Prob. 1CCCh. 9.6 - A nucleosome is composed of 146 bp or 147 bp of...Ch. 9.6 - After they have replicated and become compacted in...Ch. 9.6 - Prob. 1BCCh. 9.6 - Which of the following is the correct order for...Ch. 9 - What is/are the main component(s) of chromosomes?...Ch. 9 - Prob. 2TYCh. 9 - Prob. 3TYCh. 9 - Prob. 4TYCh. 9 - Of the following statements, which is correct when...Ch. 9 - Prob. 6TYCh. 9 - Prob. 7TYCh. 9 - Prob. 8TYCh. 9 - Prob. 9TYCh. 9 - The conversion of euchromatin into heterochromatin...Ch. 9 - What are the four criteria that the genetic...Ch. 9 - What are the key features of DNA that allow it to...Ch. 9 - PRINCIPLES A principle of bioloy is that structure...Ch. 9 - Prob. 1CBQCh. 9 - Prob. 2CBQ
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- In the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one light strand and one heavy strand ("hybrid") after one generation of growth in medium containing 14N? After two generations of growth in a medium containing 14N? What fraction of hybrid DNA is expected after n generations of growth in a medium containing 14N?arrow_forwardThe Meselson-Stahl experiment provided strong evidence that DNA replication was conservative, by alternately growing bacteria in medium with heavy 15N and light 14N. If DNA replication were dispersive, what result would Meselson and Stahl have observed after the first round of DNA replication in light nitrogen? Group of answer choices Two bands, one at the location for pure 15N and one at the location for pure 14N. One band, located half way between the locations for pure 15N and pure 14N. Two bands, one at the location for pure 15N and one located halfway between the locations for pure 15N and pure 14N. None of these Three bands, one at the location for pure 15N, one at the location for pure 14N, and one at a location halfway between.arrow_forwardThe chromosome of E. coli contains 4.6 million bp. How long will it take to replicate its DNA? Assuming that DNA polymerase III is the primary enzyme involved and that it can actively proofread during DNA synthesis, how many base pair mistakes will be made in one round of DNA replication in a bacterial population containing 1000 bacteria?arrow_forward
- In gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of the electric field? The positive pole? Why are these attractions and repulsions important to the process?arrow_forwardIf DNA replication followed the dispersive model of replication, how would the outcomes of the Meselson-Stahl experiment change? Describe the composition of DNA samples after one and two rounds of replication, and how this is different from the findings of the original experiment.arrow_forwardDescribe the Meselon -Stahl experiment Include how they distinguished new from old DNA strands, what the centrifuge was for, and how it showed semi-conservative replication.arrow_forward
- Approximately how many high-energy bonds does DNA polymerase use to replicate a bacterial chromosome (ignoring helicase and other enzymes associated with the replication fork)? compared with its own dry weight of 10–12 g, how much glucose does a single bacterium need to provide enough energy to copy its DNA once?arrow_forwardIf DNA replication were dispersive instead of semi-conservative how many "bands" would Meselson and Stahl have expected to see in their experiment after 4 rounds of replication in "light" nucleotides?arrow_forwardRefer to Figure, which presents the Meselson–Stahl experiment. DNA molecules can be denatured by high pH, as well as by heat. Suppose that the CsCl gradient centrifugations were run at pH 12, conditions under which DNA strands separate. Sketch the gradient profiles expected for each of the four samples depicted in the figure.arrow_forward
- In terms of new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reactions?arrow_forwardIn the Meselson–Stahl experiment thatestablished the semiconservative nature of DNA replication,the extraction method produced short fragments of DNA. Whatsort of results might have been obtained with longer piecesof DNA?arrow_forwardIs it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semi-discontinuous in in vitro replication?arrow_forward
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