Elisa Allen Essay

Diabetic retinopathy is induced by changes in retinal vasculature due to diabetic pathologies. Studies have shown endocrine fibroblast growth factor-21(FGF21) and non-endocrine fibroblast growth factor-1(FGF1) have therapeutic potential by increasing insulin sensitivity in vivo through unresolved mechanisms. To investigate this effect within the retina, exogenous FGF1(0-100ng/mL) and FGF21(0-100ng/mL) were administered to the murine-photoreceptor cell line, 661W. FGF1(p<0.05), with a high dose restorative

Enzyme-linked immunosorbent assay (ELISA) Lab Report Abstract The enzyme-linked immunosorbent assay (ELISA) is a common laboratory technique used to measure the concentration of an analyte (usually antibodies or antigens) in solution. In the practical anti-BSA antibodies that had undergone serial dilutions were added to a BSA solution in an ELISA plate with goal of seeing how the concentration of anti-BSA antibodies would affect the colour change of the BSA solution. The results clearly

Shrutika Mintri/ E2 Date : 09/17/2014 Lab Report #1 Project Title: BCA Protein Assay Purpose: BCA (Bicinchoninic acid) Assay otherwise known as the "Smith Assay" has the fundamental purpose of determining the protein concentration of the two given unknown proteins in the sample solutions. The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed

proteins, Apical membrane protein 1 & Internal trancribed spacer and B. bovis Spherical body proteins 4. The serological diagnostic tools, including the indirect fluorescent antibody test, the Indirect enzyme-linked immunosorbent assay, a competitive ELISA will be conducted to detect Babesia antibodies in serum of naturally infected cattle based on Babesia lysate, Cyanogen bromide-activated Sepharose 4B, affinity purified antigen and expressed,

Laboratory diagnosis The clinical signs of BVDV infection are highly variable and there are many factors that affect severity of infection like viral strain, age of affected animals, immune status, reproductive stage and mixed infection. Therefore, diagnosis based on history, clinical signs and postmortem examination should be considered only as preliminary diagnosis. The accurate and confirmatory diagnosis of BVDV infection depends only on laboratory diagnosis. 1- Virus isolation BVDV isolation

Clostridium botulinum produces toxin called Botulism neurotoxin (BoNTs) which is responsible for botulism. There are seven serotypes BoNTs of which only A, B and E are responsible for human botulism. These toxins bind to presynaptic nerve terminal of neuromuscular junction thus disrupting cellular communication resulting in muscular weakness and paralysis. Various ways were discovered for treating botulism which involve producing murine monoclonal antibodies against BoNTs. But if such antibodies

Budget Justification The budget requested in this competitive renewal application is about 20% more than the current budget. The primary reason for this increase is due to an increased expenditure in breeding animals and animal service charges as we expanded the use of animals in the proposed grant. In the past 4 years, employee salaries at the UT Heath Center were increased by about 10%. This contributed a minor fraction of the increase. We used a 3% cost escalation in preparing the budget for

The earliest microfluidic devices were made mostly made from silicon since the technology for patterning silicon wafers had already been developed by the electronics industry for its usage in microelectronics and micro electro mechanical systems (MEMS). Silicon however has the following disadvantages: (1) It is not optically transparent in the wavelength range used for optical detection; (2) Its conductive properties make it unsuitable for applications that involve electro kinetic flow to transport

tissues especially fibroblast17 , White Mineral Trioxide Aggregate was used in the present case to seal the fracture line enabling lesser possibility of leakage of dual cure composite across the fracture line. Even enzyme-linked immunosorbent assay (ELISA) has evidently demonstrated the stimulatory effect of Mineral Trioxide Aggregate on the production of bone morphogenetic protein (BMP-2) and TGF-1 by fibroblasts of human periodontium18 The present case report concluded

tissue will be extracted from the thoracic aorta and snap-frozen by liquid N2 until later use. Inflammation In order to determine inflammation within the PVAT and plasma, methods of data collection will utilize quantitative real time RT-PCR and an ELISA kit to assay lipid serum measurements9,15 as to determine cytokine and adipokine concentrations. Inflammatory markers The inflammatory markers presented below have the capability of influencing one another and are intertwined through mechanisms that