Absorbance

through and some of it will be absorbed. This is important because based on the amount of light absorbed by the solutions we can calculate their concentrations. This important and simple principle is known as Beer’s Lambert law; which states that the absorbance is proportional to the product of the path length, the molar absorptivity and the concentration of a solution. The product of molar absorptivity times path length gives the value of K, which is the slope. (Equation below) A = Ɛ.Ɩ. C K = Ɛ.Ɩ. A=K×C

(1-5) and to the blank, the result of absorbance was read at 595 nm. Result | Protein Concentration(µg/ml) | Absorbance(595 nm) | Tube 1 | 120 | .922 | Tube 2 | 60 | .557 | Tube 3 | 30 | .377 | Tube 4 | 15 | .180 | Tube 5 | 7.5 | .082 | Unknown: .759 | The ending result of this experiment confirms that as five test tubes are lined up with the varying level of absorbance, different results in the level of absorbance will appear as well, this is visible

instrument (r2 = 0.994). The absorbances for the Cary 60 were more accurate than the absorbances for the Ocean Optics when compared against accepted absorbance values (Appendix C) for the absorbance accuracy and linearity portion of the experiment (tables 2 and 3). These differences can be attributed to stray light and background corrections. Because the Ocean Optics sample holder is exposed to outside light, it is more prone to scattered light error, which inflates the absorbance expected. The Cary 60

concepts used from Beer-Lambert’s Law are the absorbance and concentration of the tested solution. The equation is A=EbC. A equals absorbance, E is the constant when identifying the species measuring the wavelength, b is the thickness of the solution, and C is the concentration of the solution. E and b are both constant, which makes the concentration and the absorbance directly proportional. Results resembled the standard curve and the connection of absorbance and concentration was used to estimate

concentration of a solution and the amount of light that the solution absorbs. Moreover, another objective of this experiment was to gain an understanding about the mechanical components of a spectrophotometer and to successfully use the device to measure absorbance or transmittance values. Lastly, another goal of this lab was to understand the concept of calibration curves and the process of interpolating data. Essentially, this lab utilized all of the processes mentioned above to determine the percentage

Tyrosinase: The Sun Sponge Hannah Farrow Elaine Burnett, Jessica Hernandez, Adryan Powell Biology 1333-003, Michael Gillis, February 15, 2017 In a constantly changing world, that is entirely dependent on plant life to sustain the atmosphere and ecosystem, it is worth noting exactly how plants live, what mechanisms they use to grow and thrive and what outside forces affect them in both negative and positive ways. Tyrosinase is best described as the enzyme responsible for the darkening of pigment

Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution, which are coloured, absorb the light depending on how concentrated it is and this produces an electronic reading from

According to the experiment results the hypothesis was proven to be correct. The diabetics did indeed have a higher absorbance level than the non-diabetics. It was made clear that the rate at which a diabetic digests their meal is slower than a non-diabetic. It proves that fact that without sugar, the body could not function properly because there would not be enough glucose in the body to provide energy in order to make ATP. Without the main ingredient to make ATP the body would die. This fact leads

spectrophotometer to measure the absorbance of each. Absorbance of a set of standard known protein concentrations from a range of 0.125 mg/ml to 2.000 mg/ml were measured using the spectrophotometer at a set wavelength and this data was plotted. The equation generated from the best fit line of this data was used to determine the unknown concentrations of the three different samples. These values were then compared to the protein concentrations obtained from the corresponding

conducted there were baseline experiment, temperature, pH, 10X substrate, Inhibitor, and half the amount of enzyme. The baseline experiment is the control group. If the time increases over a period a time then turnip hydrogen peroxidase will increase absorbance. The temperature can have a major impact on an enzyme. According to Campbell Biology author Reece etc. 2011 “The enzyme reaction will increase as the temperature increase with the increasing temperature….substrates collide with active sites more