Agar

Nutrient Agar To observe the colony morphology, the original unknown culture was streaked for isolation on nutrient agar using the quadrant streaking technique, inverted, and incubated at 37° C for 48 hours. This allowed for observation of the colony morphology. Separation of Gram-positive and Gram-negative The original unknown culture was streaked for isolation on Columbia CNA agar and MacConkey agar using a quadrant streak, inverted, and placed in a 37° C incubator for 48 hours. CNA agar contains

The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii, regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods, optimum conditions and the

each to be plated on 0.4 g potato dextrose agar (PDA). The PDA (10.26% potato extract, 51.28% glucose, 38.46% agar, 35.5366 Nm cycloheximide) uses potatoes as a source of nitrogen, potassium, and glucose as the carbon source, while cycloheximide is used to inhibit fungal growth. Of each dilution 0.1 ml was plated on PDA and spread with the hockey stick method. Each plate was then labelled with the dilution factor and placed in the incubator at 37°C for 24-48 hours dependent upon growth. A seventh

bacterial species. The tube that was chosen, tube number two, was brought to the vortex mixer so that the organisms were thoroughly mixed. Then the two organisms were isolated by using the streak plate technique.1 Using aseptic technique, the two nutrient agar streak plates were made and incubate at two different temperatures: 25 C and 37 C.1 The streak plates were incubated for 48 hours before being put into the refrigerator for five days. The streak plates then were observed and characterized based on

5.5 Antimicrobial Activity The antimicrobial agar diffusion assay was performed according to disc diffusion method against four bacterial strains; Bacillus subtilis , Shigella flexneri, Escherichia Coli ,Enterobacter cloacae and four species of fungi Saccharomyces cerevisiae, Aspergillus candidus, Aspergillus niger and Pencillium Species.Potato Dextrose Agar for fungus and NAM(Nutrient agar media) for bacteria was prepared according to the accurate composition and immediately after autoclaving,

Procedure: The blood agar petri dish was stored in a refrigerator prior to the start of the experiment. Before the experiment could begin, the agar was left to sit in order to allow it to return to room temperature. Using a marker, four test tubes were then labeled A, B, C, and D. Using a syringe, 5 ml of whole milk was measured and poured into each test tube. Then, small samples of the e. Coli specimen were added to test tubes B, C, and D using a sterilized paper clip which was bent into the shape

MacConkey Agar plate Extremely similar to Eosin Methylene Blue Agar, MacConkey Agar is a selective and differential media used to isolate organisms based on their lactose fermentation ability. It contains lactose, bile salts, neutral red, and crystal violet (Leboffe, A Photographic Atlas for the Microbiology Laboratory). Just like the Eosin Y and Methylene Blue dyes in the EMB plate, the bile salts and crystal violet in the MacConkey plate encourage the growth of Gram-negative bacteria while preventing

Introduction: Agar is a polymer made up of subunits of the sugar galactose, its also a part of the cell walls of several species of red algae that are found in eastern Asia and California. Agar is dissolved in boiling water and cooled, because of this laboratory agar looks gelatinous. Other less well-known uses include serving as a thickening for soups and sauces, in jellies and ice cream, in cosmetics and for clarifying beverages. Agar is used for culturing bacteria, its more beneficial than regular

Vanilla coffee jelly Agar agar is a jelly-like substance from red algae discovered in 1650 in Japan. Finding its amazing properties as well as inspired by runawayrice.com, I have made a tasty dessert for coffee addicts. As I love real French vanilla coffee, the following recipe is to make coffee and cream jelly. It is refreshing, mellow, cold, and healthy, flavoured with vanilla and chocolate, excellent for hot summer party. See the recipe bellow. What is agar agar ? Agar agar is derived from polysacharide

media unique. Whether nutrient agar and broths, minimal agar, chemically specific agar made of a combination of certain chemicals or chemically undefined agar which is made up of composites of different materials, the media used for growing and observing microorganisms is important and within each test, experiment, or observation, the precise media used is specifically chosen. The lab this semester has worked mostly with growth media, most commonly, TSA or tryptic soy agar. Growth media are media used