Agar plate

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  • The Agar Of Agar Plate

    1096 Words  | 5 Pages

    where that’s at the Agar in the plate shifted to the left side of the Agar plate. As a result, it was a failure. This was an indication to start the experiment portion of lab number 2 over again. The results for lab number 3 was successful. Look at figure number 1.0 on page 17. The plaques are distributed nicely throughout the Agar plates. The plaques are also opaque, large, and without any white spots known as contaminates. Lab number 5 revealed one successful Agar plate with a minor contamination

  • Pglo Lab Report

    1872 Words  | 8 Pages

    solution was added to each microtube, with use of a sterile pipette. Afterwards, the pipette was placed in a bleach solution and the microtubes were placed into the foam rack and onto crushed ice. Next, a colony of bacteria was removed from the starter plate with a sterile loop and thoroughly mixed within the +pGLO microtube. This step was repeated with a second sterile loop and the -pGLO microtube. After their use, all loops were placed in the bleach solution for decontamination and the microtubes were

  • The Effect of Environmental Factors Such as Temperature, Osmotic Pressure, Oxygen Concentration and Ph on Microbial Growth and Survival

    2280 Words  | 10 Pages

    onto the respective sections. The plate was then incubated at 37oC for 48 hours. The process was then repeated on nutrient agar plates with 5, 6.5 and 10% salt. Experiment 5: Effect of oxygen concentration on bacterial growth A fresh pipette was used to transfer 0.5ml of broth culture of E. coli, to be inoculated, into a tube of molten agar previously boiled to drive off oxygen. The tube was then rolled to distribute the bacteria and allowed time for the agar to harden. The tube was incubated

  • The Effect Of Standard Curve Developed Using A Concentration Gradient Of Titration

    988 Words  | 4 Pages

    properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values

  • High School Bacteria Experiment

    743 Words  | 3 Pages

    and we thought that the surfaces used more would have the most bacteria. For this experiment we used agar plates, cotton swabs and distilled water in order to see how much bacteria was on each surface. Cotton swabs were wet with distilled water and then the swabbed ws wied on a 2 inch space of the surface and twisted to cover the entire swab.once we swabbed the surface, we wiped the swab on the plate in a zigzag motion holding it upside down. The girls bathroom sink handle had 389 bacteria, and the

  • Manipulation of Bacteria Essay

    3385 Words  | 14 Pages

    MacConkey agar plate. The first part of the experiment involved the methods of manipulating, identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source, nutrients, pH, and temperature’. Therefore, MacConkey Agar being the

  • Examples Of Selective Media In Microbiology

    1544 Words  | 7 Pages

    Eosin Methylene-Blue agar used for the growth of Escherichia coli. These bacteria are commonly found in places of fecal contamination (CDC). A control group of Escherichia coli was used as well as swabs from the student’s phone and hand. Another type of media used in this experiment is Differential/Selective media. This media makes detection easier after the incubation period has occurred. An example of differential/selective media used in this experiment was Mannitol Salt agar used to isolated the

  • Assignment 2: Identifying Bacteria

    2317 Words  | 10 Pages

    UNKNOWN 65 Identifying bacteria is beneficial for several reasons. In the food industry, the discovery of bacteria and other microorganisms helped develop processes to prevent food spoilage, such as pasteurization, and bacteria is used to make certain foods, such as cheese, butter, vinegar, and alcohol (1). The discovery of bacteria that break down waste products is beneficial to the waste treatment industry, and the discovery of nitrifying, ammonifying, and nitrogen-fixating bacteria are important

  • Streak Plate Essay

    654 Words  | 3 Pages

    Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the loop. For that reason,

  • Microbiology Unknown Lab Report Essay

    2396 Words  | 10 Pages

    this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for?