Agar plate

first section of SBA plate  After that is down sterilize loop and continue streak plating technique for next two sections o Perform same steps for MSA using a new swab o Incubate plates for 24 hours at 35 degrees Celsius PERIOD B – Individual work • Part 1: Selective and Differential Media o Study/observe all three media plates for growth o Write down observations  what growths happened and changes in the media • Part 2: Isolation of Bacteria (mixed culture) o Observe plates and compare for differences/similarities

On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of bacterium. Once we were given our broths, we had to streak two Nutrient Agar plates with the mixed culture tube by using a loop. After I streaked my Nutrient Agar plates, I decided to perform a gram stain. First I flamed the loop and set it aside to cool. After I flamed the loop I prepared two slides with a drop of water in the middle. Once the loop was cool I dipped the

UNKNOWN 65 Identifying bacteria is beneficial for several reasons. In the food industry, the discovery of bacteria and other microorganisms helped develop processes to prevent food spoilage, such as pasteurization, and bacteria is used to make certain foods, such as cheese, butter, vinegar, and alcohol (1). The discovery of bacteria that break down waste products is beneficial to the waste treatment industry, and the discovery of nitrifying, ammonifying, and nitrogen-fixating bacteria are important

66). The two TSA plates were incubated both aerobically and anaerobically because it displayed how oxygen affected the growth of each organism. Both aerobically and anaerobically incubated plates displayed identical white, convex elevated isolated colonies with entire margins and glossy, convex elevated isolated colonies with entire margins. Therefore, this

Introduction Microbiology is the study of microorganisms; whether it is bacteria, fungi, algae, protozoan, viruses or archaea (Leboffe & Pierce, 2006). Microbes come in all different structures and classifications. Before the 17th century the idea or microbes existed, but was never proven until a scientist provided something that allowed us to see what the naked eye alone could not see (Pelczar, 2015). It all began when Anton van Leeuwenhoek invented a microscope and began observing objects under

was diluted into a tube and poured on the plate. The first inoculation was the original phage suspension and from there we diluted it three more times in 9-ml dilution blank test tubes. Once the test tubes were inoculated .5-ml of each dilution was placed in soft agar tubes quickly, so that it would not solidify, and then were poured on the trypticase-soy agar plates. The TS agar plates were left to incubate for 6-10 hours. Once done incubating each plate was then counted to see how many plaques had

Phase I-Phenotypic characterization 1. Collection of microbial isolates from various sample, characterization, identification and performing antibiotic susceptibility testing. 2. Performing Metallo Beta Lactamase (MBL) screening and confirmatory tests. 3. Testing carbapenemase production phenotypically. 4. Testing microbial isolates against higher and multidrug antibiotics. Phase II-Molecular Characterization 1. To identify the type of carbapenemase and MBL genes by PCR 2. To identify the expression

The microbe that I received was on plate B section 5. I began the project by creating pure cultures of my microbe on a sterile nutrient agar plate, a nutrient agar slant and an agar broth by using Alderson’s procedure for aseptic techniques (Alderson, 2015, p. 53). After inoculating the media with my microbe, I incubated them at 37°C for 48 hours to allow for growth. After 48 hours, utilizing Alderson’s technique for making smears from solid cultures I created 3 smears (Alderson, 2015, pp. 93-94)

properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values

transformation efficiency was calculated by dividing the total amount of DNA on the agar plate in μl by the number of cells growing on the +pGLO LB/amp/ara plate. During the purification section of this lab, the LB/amp/ara agar plate was examined for well-isolated green colonies and the LB/amp plate was observed for white colonies with space between each other. These colonies were circled on the outside of the plates using a marker. Next, two 15 milliliter culture tubes containing 2 milliliters of