Agar plate

a)Bacterial inoculum preparation (isolation and culturing) (95,97): - A pure standard Enterococcus Faecalis strain (ATCG 29212) was isolated and sub- cultured in blood agar plates and incubated at 370c for 24hours. - A pure single E.faecalis colony was isolated from the cultured plates and gram stained to confirm the colony growth under microscope.(Fig.12) - The isolated colony was then inoculated with nutrient broth and incubated again at 370c for 24hours in anaerobic conditions. The bacterial growth

grow in discrete colonies and to isolate pure culture from the mixed culture we used an agar streak plate. We also sterilized the inoculating loop in the bunsen burner by putting it in the flame until it is red hot, then chose an isolated colony from the agar plate culture colony and spread it over the first quadrate then we repeated this till we covered all the areas of the plate. After that, we leave the plates for a week. this allowed us to determine how many colonies were presented. Serial dilutions

period the samples were mixed well will a vortex. We then piped 1ml of the first dilution into the second dilution and mixed thoroughly with the vortex. This process was repeated with remainder of the dilutions.. Streak plates were prepared in petri dishes filled with premade nutrient agar and 100µl of the third, fourth and fifth dilutions. We streaked an additional

Finding the Minimal Inhibitory Concentration: The Process and the Importance Melissa Bañaga San José State University Abstract The purpose of this project was to determine the minimal inhibitory concentration of our given antibiotic called tetracycline. Tetracycline is a bacteriostatic agent and instead of directly killing the bacteria, it prevents the growth mainly by inhibiting the synthesis of proteins. We hypothesized that the minimal inhibitory concentration for our test would

my finger on the agar plate, the bacteria will grow fast and will start to form colonies of bacteria. After the bacteria forms colonies, you won’t need a microscope to be able to see the bacteria. This will show us how washing your hands can keep you from getting sick or spreading a sickness you already have. 2. Materials: Paper towels Soap Incubator Wax Pencil Agar Plate Parafilm Sealing Film Student Data Sheet 3. Procedure: Day One: With your partner, get an agar plate and wax pencil.

the 10 minutes, get the LB agar and LB+AMP agar plates ready. Mark your plates with the transformation tube mixture to use (+ or -), the lab group names, and the date on the top of the dishes. 12. Add 100ul of the pGLO transformation cell mixture to the center of the agar surface of the corresponding LB agar and LB+AMP plates. 13. Use a sterile plastic loop to distribute the cell suspension evenly on the plate by “skating” the loop back and forth across the LB agar plate several times. 14. Use

membrane disruption, and protein coagulation, these along with Hydrophilic Viruses (viruses which are mixed with another liquid, eg; water) also can also be resistant. Although E. Coli is hydrophobic, if any air transmitted viruses were to settle on the agar, the results would be extremely altered. Conclusion: My hypothesis lined up with the results, but as there

better understanding of the unknown microorganism in terms of the physiology, morphology, motility, and antimicrobic sensitivity it is characterized with. Indole production, hydrogen sulfide, and the colony morphology on the Eosin-methylene blue (EMB) plate, were the critical results that led to the conclusion that the organism was E. coli. In the indole production test, E. coli was one of two organisms,

the skin microflora lab, the materials that were used were: plates of S.aureus, S.epidermidis and S.pyogenes, a DEMO Mannitol Salt agar (MSA) +/- plate, simple stain reagents, 3% hydrogen peroxide (H2O2), plasma, sterile water and swabs, a Phenylethanol agar (PEA) plate, a sterile inoculating loop, a MSA plate and microscope slides. The first objective of this lab was to obtain a sample of our individual skin flora. First, a PEA plate was obtained and divided into two section labeled as moist/oily

results. Exercise #1 Materials Needed: A Nutrient Agar Petri Plate Sharpie Marker Fingers and/or Thumb filled with bacteria Sink Soap Paper Towels Incubator rack marked 37C Procedures: In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,