Agar plate

Introduction- The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique

make single line inoculations on both an Eosin Methylene Blue (EMB) and a Salmonella Shigella (SS) plate. Each plate was divided by a a line in half so that unknown B was inoculated on one side and unknown C was inoculated on the opposite side. The EMB plate contains lactose and dyes eosin and methylene blue. The media is used to differentiate between lactose and non-lactose fermenters. The SS plate also contains lactose in addition to bile salts and brilliant green (dye) to select for species of

Abstract and Introduction Microorganism identification plays a very critical role in the medical significance of modern medicine. One example of reasoning behind it can be for the detection of a disease causing organism in a patient, so it can be certain that it will be appropriately treated. Numerous species of bacteria can appear to share identical morphology (spirilli, cocci, rods), when looking through a microscope. There are many methods and biochemical tests that can be performed to differentiate

specimen streaked on a plate could come in a several of forms, such as solid or liquid samples and cotton or foam swabs. Material containing possibly infectious agents should be handled in the lab using proper bio safety procedure. Remove the test tube cap; Insert the loop into the culture tube and remove a loopful of broth. Replace the cap of the test tube and place it back into the test tube rack. The lid of the agar plate has to be opened just enough to streak the plate with the inoculation loop

There are numerous influences that can affect the growth of a bacteria. These influences include the presence of oxygen, pH, the accessibility of water, and temperature. The abundance or lack thereof for each of these factors can determine whether a bacterial cell is able to propagate or if it will ultimately die. Additionally, the intensity of these factors can also determine the amount of growth seen. In order to observe this relationship one of the influential factors was chosen and the rate

student must retrieve a agar plate and mark the plate in sections with a labeling pen. After labeling the agar plate, the group must then get 250 ul of Arthrobacter. To make the bacterial lawn, you must obtain 3 mL of top agar and mix it with the 250 ul of arthrobacter using a 5mL pipette. With the pipette, a student should mix the two liquids by bringing it up and down. After this has been achieved, students must then quickly place the mixture inside the agar plate and tilt the plate side to side to equally

Neisseria or Streptococcus were present in these locations. The features that make it easy to identify these two types of bacteria are that an oxidase test can be done for the Neisseria bacteria and using a blood agar to identify beta hemolytic species of the Streptococcus. Using the agar plates, the colonies were put through a few tests to see if the bacteria grown was the wanted pathogens or not. A prediction that Staphylococcus aureus would not be found in the nose of the group member, because many

be identified through biochemical tests. After preparing a working plate, a Gram staining test, a catalase test, and a RBC hemolysis test were done for the identification. The Gram staining resulted in the unknown bacteria being a Gram-positive coccus because of the blue coloration and a spherical shape under the microscope. The catalase test was negative because the bacteria did not utilize catalase to breakdown H2O2. The blood agar used for RBC

Hydrolysis of starch Materials: 1. Starch agar plates 2. Broth agar cultures of B.subtilis and E.coli Procedure: 1) Starch plate was streaked with E.coli in for sections and repeated for B.subtilis bacteria in other starch plate. 2) The plates were secured with parafilm, labelled and inoculated at 37oC for 24 hours. The following day 1) The plates were tested for starch hydrolysis by flooding the pates with Gram’s iodine. 2) The plates were examined and the colonies that showed

properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values