Agar plate

INTRODUCTION Bacteria are unicellular microorganisms too small to see with the naked eye that exist in virtually all environments in the world. They inhabit various environments such as dirt, water, and inside the human body (Bacteria, What, 2015). These organisms that have the capacity to live as either independent organisms or parasites (MedicineNet.com, 2012) are remarkably powerful and complex in nature as they can be both vital and detrimental to the health of an organism. As some bacteria

from crossing over is easily seen (Davidson). The organism Sordaria Fimicola is a good example of this process because it is easy to grow on agar plates and because they are easy to be seen when looked at through a

Microorganisms can easily be found in water. In third world countries, this can pose a huge threat, because lots of people can get sick from drinking water. SODIS is a method used by many in undeveloped countries as means of attaining microbe-free water. It involves allowing water to stay exposed to sunlight in order to kill whatever microbes are living in it. For this experiment, microbial diversity was determined by collecting a sample of water of different from shaded water and water directly

Bacteria can be found on numerous locations of the human body, while some are beneficial to human health others are not. Upper respiratory tract infections for example can be cause by pathogenic bacteria. Left untreated, respiratory infections can be harmful or even fatal in some cases. Therefore, identifying the cause of the infection is paramount. Knowing the target would allow the correct treatment methods to be utilized, such as the use of an effective antibiotic. The purpose of the experiment

rpm) for 48 h. After this enrichment, suspensions in physiological saline were used to inoculate duplicate modified Wickerham agar (supplemented with 100 mg/L chloramphenicol) and modified Sabouraud dextrose agar plates with yeast and fungi, respectively. All media were modified by using filtered seawater instead of distilled water to dissolve all ingredients. The plates were

following sections discuss different materials and methods used to isolate and identify the unknown organism 52. Section II – Material and Methods Unknown 52 was initially grown in a Tryptic Soy Agar (TSA) plate, which is an undefined medium where the exact composition of the nutrient was not known. TSA plate was incubated for 24 hours at 35 oC which showed isolated colonies that were small, white, flat smooth margins, round, shiny colonies on the medium (1). Microscopic Examination Unknown 52 was

Next, using proper inoculation technique, which is heating the loop with a Bunsen burner until it is red hot and cooling it in the edge of the medium agar, grab a small but barely visible amount of Bacillus Cereus and drop it into the nutrient agar slant. Heating the tube in an 80-degree Celsius water bath should kill all of the vegetative bacterial cells in the sample, but the endospores within the sample should remain since they are resistant to

sample was transferred and inoculated to plate count agar. For coliform count, 1mL of milk sample was transferred and inoculated to MacConkey agar (MAC). All plates were incubated at 37°C for 48 hours. Every single colony on plate count agar are counted and this indicates number of microorganisms present in milk samples which is 6.7×105 cfu/mL. Based on unpasteurised milk standard code 1.6.1 (ANZFA-2009), acceptable microbiological level of standard plate count (SPC) is 2.5×104 cfu/mL. Total viable

looking for the sulfur reduction, indole production, and motility of the organism. First, a SIM media tube is prepared, and then label the SIM tube with your initials and the bacteria you’re using. Next, use a needle to pick an isolated colony from the plate. Then, inoculate the tube by making a single straight stab down the middle

transferring the yeast. The mold must be cut from the petri plate and placed on top of the drop of lactophenol cotton blue already placed on the microscope slide. After it is covered it may be studied under the microscope. 3. State the scientific name of the yeast studied in the laboratory. Saccharomyces cerevisiae is the scientific name of the yeast studied in the laboratory. 4. Name the medium upon which the mold was cultured. Sabouraud agar is the medium upon which the mold was cultured.