Agar plate

A Dairy Problem: Listeria monocytogenes I. History One of the earliest detections of Listeria was in 1924 by E.G.D. Murray from rabbits. He isolated Gram-positive rods from a rabbit’s blood in the laboratory. He was unable to classify it with other bacterial genus, thus he decided to call this new species Bacterium monocytogenes. However, only after 1940 that this bacterium was given the genus name Listeria by J.H.H. Pirie. He was able to classify it due to its catalase-positive, Gram-positive rod

meningitidis and the diseases that it is responsible for are diagnosed by blood samples or a spinal tap to retrieve cerebrospinal fluid samples. Since the bacteria grows fastidious and is oxidase positive, it is preferred to grow it on a blood agar plate or a chocolate agar plate at temperatures ranging from 35-37 degrees Celsius. It can be identified using the Kovac’s oxidase test and if a positive result is found it should be followed with a carbohydrate utilization test. If this too shows N.meningitidis then

lifted up the loop to look for what appears to be a “stringing” affect which means it’s confirmed that it is gram- negative species. Next, I created a streak plate using nutrient agar so that I could see pure culture of my organism. I aseptically obtained a loop full of my organism and gently inoculated one quarter of the nutrient agar plate by running the loop back and forth across the surface. I then flame sterilized the inoculating loop, allowing it to cool for 10 seconds, and then streaked the

therefore its name had been changed one final time to Morganella (its own genus) morganii. While testing M. morganii, findings show that it has its own special characteristics that differ from the usual Proteea. M. morganii does not swarm on a nutrient agar plate like the typical Proteus would. It also does not produce the black precipitate found in Hydrogen Sulfide gas tests. M. morganii produces phenylalanine deaminase, which is the enzyme that wipes out the amino group, resulting in a phenyl pyruvic acid

shorter generation time? Give a detailed explanation! 5) Your TA gives you an E.coli culture that has a concentration of 2000cells/ml. He/she tells you to evenly spread 25µl of this culture on an LB agar plate and another 25µl on a GMS plate. Both plates are incubated at 37oC. What do you expect to see the next day and why? Give a detailed explanation! 6) You want to determine what the optimal pH is for E.coli to grow. How would you set up the experiment

Neonatal contamination can be obtained • In utero transplacentally or through ruptured membranes • Within the delivery canal at some point of transport (intrapartum) • From outside resources after delivery (postpartum) In utero contamination, that can arise any time earlier than delivery, results from overt or subclinical maternal infection. Results depend on the agent and timing of contamination in gestation and encompass spontaneous abortion, intrauterine boom restriction, premature delivery, stillbirth

Staphylococcus epidermidis. The hypothesis is that the hand sanitizer will be able to eliminate S. epidermidis effectively. The use of alcohol to sanitize needles and other surgical items is the reason behind the hypothesis. Obtaining a Trypticase Soy Agar (TSA) Petri dish, a dish that has a TSA layer, a gelatin-like substance that remains semi-solid at room temperature. Also used was a sterile swab to move the bacteria to the TSA dish. A small bottle of S. epidermidis culture, which is a bacteria

of Vibrio cholerae 23(52.3%) male and 21 (47.7%) female. Regarding to the serotyping of V. cholerae this study found that all isolates have only Inaba serotype. The virulence factors were detected in all isolates was produce β-hemolysis on blood agar. The highest one appeared at the (11-20 and 21-30) years age group with 29.6%, followed by others at age groups (>60) years and (41-50) years with 11.1%. The low isolation frequency was recorded at (51-60) years in a percent 3.7%. The results

chemicals and solvents used in the study were of analytical grade and were procured from popular chemical firms (SRL and S.D. Fine Chemicals, Mumbai, India). Biochemical and physiological test kits were obtained from Hi-media, Mumbai, India. The TLC plates were purchased from Merck India limited. 3.2. PREPARATION OF MEDIA AND REAGENTS The Mineral Salts Medium (MSM) was prepared as per Brilon et al., (1981) with some modifications. The MSM contained of following constituents (g/l): Na2HPO4.2H2O (12.0)

fixation. Pea is liable to be attack by many bacterial, fungal, viral, nematode diseases in addition to physiological disorder. However, fungal diseases, especially rust is considered one of the major destructive diseases affecting the crop yield (Hagedron, 1984 and Kraft, and Pfleger, 2001), especially in the north and middle parts of the Delta in Egypt and several countries in the world (Abada et al., 1997; Gupta and Shayam, 1998 and Parilli et al., 2015). The fungus Uromyces pisi is a heteroecious