Agar

contributes to the biogeochemical cycle and without bacteria and fungi we could not continue our existence on our planet “earth. METHODS AND MATERIALS MATERIALS 1 Becker 1 Nutrient agar plate 1 pair plastic gloves 1 Potato agar plate 1 Staining tray 100ml of distilled water 2 MacConkey Agar Plates 3 Blank slides 3 plastic cups 6 pipettes 95% Grams alcohol Bibulous paper Boat & Scale Bunsen Burner & Flint Crystal-violet Grams Iodine Incubator of

then incubated at 37oC for 48 hours. The process was then repeated on nutrient agar plates with 5, 6.5 and 10% salt. Experiment 5: Effect of oxygen concentration on bacterial growth A fresh pipette was used to transfer 0.5ml of broth culture of E. coli, to be inoculated, into a tube of molten agar previously boiled to drive off oxygen. The tube was then rolled to distribute the bacteria and allowed time for the agar to harden. The tube was incubated at 37oC for 48 hours. The procedure was repeated

techniques, which will be used throughout this course, by aseptically transferring cultures to three types of media: broth, plate, and agar slant. Materials: • Lab coat • 10% bottle of bleach • Sharpie • Masking tape • Vortex mixer • Sterile Tryptic soy broth test tubes • Sterile Tryptic soy agar slants • Tryptic soy broth culture of Serratia marcescens • Tryptic soy agar slant culture of Serratia marcescens • Inoculating loop • Bunsen Burner • Striker Methods: Before you start any kind of Transfer

Results The Blood Agar plate showed good growth with pale yellow pigmentation and slight greening of the media indicating hemolysis occurred, shown in Figure 3. Greening of the media is usually indicative of α-hemolysis, though in some instances there may be artificial greening of the medium in organisms, which are actually β-hemolytic (Leboffe & Pierce, 2015). S.aureus is β-hemolytic, meaning it has the ability to fully break down red blood cells resulting in a clearing of the medium. The good

UNKNOWN 65 Identifying bacteria is beneficial for several reasons. In the food industry, the discovery of bacteria and other microorganisms helped develop processes to prevent food spoilage, such as pasteurization, and bacteria is used to make certain foods, such as cheese, butter, vinegar, and alcohol (1). The discovery of bacteria that break down waste products is beneficial to the waste treatment industry, and the discovery of nitrifying, ammonifying, and nitrogen-fixating bacteria are important

properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values

growth be greater than samples taken from the human body? Material and Methods Students were provided with the following equipment in a sterilized Microbiology Lab. Cotton Swabs • Distilled Water • Test Tubes with caps • Petri Dishes • Nutrient Agar • Blood Agar • Inoculation Loop • Bunsen Burner • Slides • Pipette • Compound Light Microscope • Various Reagents. Students were asked to gather samples from four sites on a college campus where it was believed bacteria would be most concentrated. Students

Use the cotton swab to streak the five labeled nutrient agar plates with the E. Coli K12 bacteria from the tube. 36. Remove a tiny bit of E. coli from the tube using the cotton swab. 37. Hold the tweezers with the cotton swab (or just the cotton swab alone if you are using extra-long cotton swabs) in one hand

Results Observing under 1000X magnification, the gram stain depicts pink bacilli, as seen in Figure 1. Figure 2 depicts the growth of a brown bacteria on a MacConkey Agar plate. Figure 3 depicts the growth of a similarly brown bacteria surrounded by a very thin clear area on a blood agar plate. As seen in Figure 4, the slant of gram-negative isolate appears mainly clear, with a slight green tint. Figure 5 shows the methyl red test, which produced no color change. Figure 6 shows the Voges-proskauer

observations in this lab were taken qualitatively (biological drawings) as there were no ways of accurately measuring the bacteria since the petri dish needed to remain sealed. During the lab, the temperature of the incubator remained constant. The same agar was also applied to the entire petri dish prior to the