Agar

Lab Report: Unknown #1 Paola Rosales 302672960 Professor Youssef INTRODUCTION: Bacteria are the smallest living organisms, they are prokaryotic and have a simple cell structure. They do not contain a nucleus, and are unicellular. Bacteria are the most abundant microorganism. They are among the earliest forms of life that appeared on earth billions of years ago. They can grow in different temperatures and many exist naturally in our human flora, such as staphylococci. Growth within different

Serial dilutions of the samples were prepared and grown on different growth media for selective culturing of the three groups. Following incubation of the plates at 37C for two days then at 4C for five days, it was observed that the colonies on the agar plates had different morphologies. Colony counts were used to determine the original cell density of the three microbial groups. Although the calculated population densities were inconsistent because of multiple countable plates, the consistent trend

Introduction Whenever there is an unknown disease caused by microorganisms, tests are usually made in order to identify the organism causing the disease. There are several tests that need to be made and they include tests such as performing a gram stain, streaking a plate to isolate colonies, inoculating a broth culture, inoculating API strip, and performing oxidase and catalase tests. Having knowledge on how to identify these tests are of high importance in the medical field so it would be to the

characteristics and morphology was Sheep Blood Agar (SBA). Figuring out specific colonies was difficult due to beta hemolysis which is seen as a zone of clearing around the colonies. The easiest to distinguish of all three medias was obviously the Tryptic soy agar (TSA). Being the general growth media, we knew what we were expecting which was to see all three bacteria to grow thus making the media not selective nor differential. We knew in the case of Mannitol Salt Agar (MSA) that it could be selective or differential

One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of bacterium. Once we were given our broths, we had to streak two Nutrient Agar plates with the mixed culture tube by using a loop. After I streaked my Nutrient Agar plates, I decided to perform a gram stain. First I flamed the loop and set it aside to cool. After I flamed the loop I prepared two slides with a drop of water in the middle. Once the loop was cool I dipped

Introduction Microbiology is the study of microorganisms; whether it is bacteria, fungi, algae, protozoan, viruses or archaea (Leboffe & Pierce, 2006). Microbes come in all different structures and classifications. Before the 17th century the idea or microbes existed, but was never proven until a scientist provided something that allowed us to see what the naked eye alone could not see (Pelczar, 2015). It all began when Anton van Leeuwenhoek invented a microscope and began observing objects under

a)Bacterial inoculum preparation (isolation and culturing) (95,97): - A pure standard Enterococcus Faecalis strain (ATCG 29212) was isolated and sub- cultured in blood agar plates and incubated at 370c for 24hours. - A pure single E.faecalis colony was isolated from the cultured plates and gram stained to confirm the colony growth under microscope.(Fig.12) - The isolated colony was then inoculated with nutrient broth and incubated again at 370c for 24hours in anaerobic conditions. The bacterial growth

Pea is liable to be attack by many bacterial, fungal, viral, nematode diseases in addition to physiological disorder. However, fungal diseases, especially rust is considered one of the major destructive diseases affecting the crop yield (Hagedron, 1984 and Kraft, and Pfleger, 2001), especially in the north and middle parts of the Delta in Egypt and several countries in the world (Abada et al., 1997; Gupta and Shayam, 1998 and Parilli et al., 2015). The fungus Uromyces pisi is a heteroecious rust

into a sterilization filter unit to filter the phages into a micro centrifuge tube. When that was completed. Proceed to add .5mL of m.smegmatis and 4.5 mL of top auger into a culture tube with the liquid from the soil sample. Pour the mixture into an agar plate, wait for the TA to harden and then invert. The next experiment

Done in the past decades, progress of new and great techniques for learning and manipulating DNA has transformed genetics. The cloning plasmid shown in figure 16.1 is one example of the tools that are now available. These techniques have permitted biologist to occur directly in the genetic fate of organisms for the first time, the ability to directly isolate and manipulate genetic material was one of the most profound change in the twenty first century, plasmid vectors (small, circular chromosomes)