Agrobacterium

genetically modify it’s hosts to incorporate beneficial novel genes? Agrobacterium tumefaciens is an unsung villain/hero that is in fact, capable of such amazing things. Agrobacteria tumefaciens is a bacterium that parasitizes plant tissue, and causes what is often referred to as “crown gall”- yet it is more the virally- reminiscent mechanism through which this occurs than the physical deformity itself that is of interest. Agrobacterium tumefaciens is motile and utilizes chemo taxis paired with its intrinsic

INTRODUCTION Agrobacterium Tumefaciens is an aerobic gram negative bacteria that is commonly known as a causal agent of Crown Gall disease in a wide array of plants. Agrobacterium Tumefaciens uses horizontal gene transfer “which is any process in which an organism transfers genetic material to another cell that is not its offspring.” (Black, Microbiology, pg13) to cause tumors in plants. It causes tumors by transferring a small segment of its DNA and inserting itself in a semi random location

Poplar is a versatile tree species that is highly amenable to vegetative propagation (Jiaxing et al. 2014), it has a rapid growth rate, which is a good model system for the transformation of woody plant species (Ali-Movahedi et al.2014). These trees are highly beneficial to our environment; their oxygen plays a vital role in the protection of soil erosion in eastern China. They are used as habitat for wildlife, soil carbon sequestration, and phytoremediation. They are able to grow in lowland and

A few years back, the idea of utilizing Agrobacterium tumefaciens as a vector to make transgenic plants was seen as a prospect and a "wish." Today, numerous agronomically and horticulturally vital species are routinely changed utilizing this bacterium, and the rundown of species that is defenseless to Agrobacterium interceded change appears to develop day by day. Agrobacterium tumefaciens is a gram-negative rod shaped microscopic bacteria firmly identified with nitrogen-fixing microorganisms which

DORN (Does Not Respond to Nucleotides 1), DORN1, L-Type Lectin Receptor Kinase I.9, LECRK-I.9 are synonymous. According to TAIR database, LECRK-I.9 is involved in protein-protein interactions with RGD-containing proteins as potential ligands, role in cell wall-plasma membrane adhesion and involved in Phytophthora resistance. The mutant dorn1 is defective in LECRK I.9. DORN1, binds ATP with high affinity and is required for: ATP-induced calcium response, mitogen-activated protein kinase activation

pattern were obtained, the relative ability of each gene to cleave coniferin was assessed. Clones containing Agrobacterium tumefaciens B3/73 DNA rapidly and completely hydrolyzed coniferin to coniferyl alcohol. Over the same period, type 2 clones were completely inactive. The different substrate specificities of clones were also evident from their ability to grow on cellobiose Agrobacterium tumefaciens B3/73 was able to use cellobiose as the sole carbon source. Escherichia coli DH5α and type 1 clones

Soybean is an important crop, with food, nutritional, industrial, and pharmaceutical uses. Soybean seeds contain about 40% protein and about 20% oil. They are also abundant in physiologically active metabolites such as isoflavones, lecithins, tocopherols and saponins, in addition to functional proteins and are used as an affordable source of foods that promote and maintain health (Sugano 2005). The genomic era is now under way for soybean, as for other many crops. Recently, a soybean genomics database

Application of Genetic Engineering on Taro Adam Mohamed 10/23/2015 Introduction The field of genetic engineering is very promising, pertaining to the future of agriculture around the world. Research in this area is allowing for more and more improvements to the various aspects of a crop such as yield, visual quality, taste, nutritional value, disease immunity, and cold resistance, as well as many other benefits. A further understanding of both the genetics of crops and how to influence

Nowadays genetic engineering uses so many techniques by the use of living cells and organism that helps to modified commercial products for specific use (Whittaker, 2013). This biotechnology splices the gene from one species of plant or animal and transfer it into another species to create various new varieties of plants that has a new characteristics that does not occur naturally.(Whittaker, 2013). Biotechnology involves alteration of DNA by introducing new DNA artificially that result in to genetically

Other genes like chvA, chvB, pscA and att regulate the formation of cellulose fibrils which are required for the first step of weak and reversible attachment. Cellulose fibrils help the bacteria to attach to the wounded parts of the plant and also anchoring to each other forming a microcolony. VirD4 protein and 11 VirB proteins make up type IV secretion system for the transfer of T-DNA and several other Vir proteins like VirE2, VirF etc to the host cell. VirD4 also promotes interaction of the processed