Alkaline phosphatase

A SPECTROPHOTOMETRIC ASSAY OF THE ACTIVITY OF ALKALINE PHOSPHATASE ON PARA-NITROPHENYLPHOSPHATE IN THE PRODUCTION OF PARA-NITROPHENOL M. Smit1 1 Department of Biological Sciences, University of Cape Town, Rondebosch Introduction: Alkaline phosphatase is a biologically important enzyme, catalysing the hydrolysis of phosphate groups in organic compounds through the process of dephosphorylation. para-Nitrophenylphosphate (pPNP) is an example of a chromogenic substrate of this enzyme, because the

Aim: to determine the kinetic parameters of the enzyme Alkaline phosphatase General Background Alkaline Phosphatase is a group of enzymes found in certain tissues (including the bile duct, bones and the liver). Based on its specific location each enzyme will present with a different structure. The highest concentration of the enzyme is found in the liver, and so is used as a liver function test

Alkaline Phosphatase: the Influence of p-Nitrophenyl Phosphate, Sodium Phosphate, and L-Phenylalanine on the Enzymatic Activity Abstract The present experiment was performed for analyzing the influence of the substrate concentration and inhibitors on the activity of alkaline phosphatase. p-nitrophenyl phosphate was used as a substrate for the hydrolytic enzyme and the inhibitory effect of sodium phosphate and L-phenylalanine was tested. The reaction rate was determined by measuring the absorbance

calculation of different kinetic parameters for alkaline phosphatase. First, it was necessary to generate a standard curve for PNP presenting the absorbance as a function of the concentration of PNP since the remaining of the experiment is based on measuring absorbance values of PNP. This standard curve is then a good tool to convert those absorbance values into PNP concentration values that can be used to study the kinetics of the alkaline phosphatase enzyme. Once the standard curve was generated

edu word count: Determination of Km and Vmax of Alkaline Phosphatase Graphs will be used with the enzyme alkaline phosphatase of the unknown in the enzyme solution to Determine Km and Vmax To Dr. Kimberly A. P. Mitchell, Editor, Liberty Journal of Cell Biology, 1971 University Blvd, Lynchburg, VA 24502. Materials and Methods Dilution: Alkaline Phosphatase The objective for this laboratory is to determine the velocity of alkaline phosphatase and make a graph of rate vs substrate concentration

Paget’s disease of the bone (PDB), also called Osteitis Deformans, is a chronic, slowly progressive skeletal condition of abnormally rapid bone destruction and reformation. PDB is the second most common bone remodeling disease after osteoporosis. The new bone is structurally abnormal, dense and fragile. The bones that are frequently affected are in the spine, skull, pelvis and lower legs. The disease does not spread bone to bone, it will stay constant in the affected bone or bones throughout

these tests is done for another reason”. NIH national resource center, 2015). The results from a blood test, is sometimes the first alert a doctor receives that a person may have Paget’s disease. The bone cell makes an enzyme known as serum alkaline phosphatase (SAP). Whenever there is a higher level of SAP than usual in the blood, it is evidence that Paget’s disease may be present. SAP in the blood that is more than two times the usual level,is a strong indication that the disease is present in the

MMR Report 1.3 Restriction Enzyme, Alkaline phosphatase Digestion 
and Gel Electrophoresis By Naga Srilekha Somu Chemistry - 429 Spring 2016 Western Illinois University Materials and Equipment: Pure plasmid pET28a, amplified 2-alcohol dehydrogenase gene (a PCR product), 10x bovine serum albumin, 10x neutralization buffer, EcoRI, nuclease free water, pET28a plasmid digested with EcoRI, calf intestinal alkaline phosphatase, agarose gel (1% agarose + 0.3μL ethidium bromide), 1x TAE buffer

values 4.2. Discussion of the significance of the results 4.3. Was the original aim achieved 4.4. Conclusion 5. References 1. Introduction 1.1. Aim To determine the kinetic parameters known as Vmax and Km of Alkaline Phosphatase. This will be done by determining and investigating the optimum pH and

wheat germ suspension, where heat was used to purify and isolate the wheat germ. In the second portion of the lab, examiners found the concentration of the wheat germ protein. In the final step of the experiment examiners found the amount of acid phosphatase in each of the tested microtubes. Materials: Step 1: • One 500µL microfuge tube • Distilled water • A bowl