Alternative splicing

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  • Essay On Cryptococcosis

    1792 Words  | 8 Pages

    CRISPR-CAS9 technique (Fig. 7). We will use a modified plasmid derived from pCAS9 that contains both the CAS9 endonuclease and the Pol III promoter for expressing gRNA. Transformation with two such plasmids, one expressing gRNA prior to the alternating splicing site and one posterior to the NEO marker in the presence of the 6 kb CIN1-L-NEO fragment as donor DNA will result in the replacement of the wild type CIN1 allele with the mutant one (CIN1-L) (Fig. 7). Once verified by RT-PCR and DNA sequencing, Cin1-L

  • Virulence Factors Of Cryptoccus Neoformans

    1020 Words  | 5 Pages

    Cryptococcus neoformans (Cn) is a basidiomycete fungus that causes cryptococcosis worldwide in immunodeficiency and healthy individuals. In this pathogen, the elaboration of virulence factors including melanin and the capsule depends on intact intracellular trafficking, an essential cellular process also required for nutrient uptake, ion homeostasis, and receptor recycling. Our previous studies have found that Cn evolves a cryptococcal intersectin (Cin1)-regulated endocytic pathway essential for

  • The Physics Of Splicing And Splicing

    1446 Words  | 6 Pages

    the optics-based method offers several advantages such as the kinetic study of splicing and splicing inhibition, study of cis–trans alternative splicing, and rapid measurement of RNA splic-ing. The kinetics of pre-mRNA splicing and the effect of isoginkgetin on the splicing kinetics of the pre-mRNA at the single molecule level were analyzed. Because of their high temporal resolution and the ability to follow the splicing of individual pre-mRNA molecules, the optics-based methods provide the evidences

  • Transcription And Translation Of Rna Splicing

    1429 Words  | 6 Pages

    a specific protein is produced. The first step in this process is called transcription where the enzymes use one of the DNA strands within a gene as a template to produce a messenger RNA or mRNA. The next step in the process is translation. RNA splicing is an important step in creating the mRNA that is involved in protein synthesis in

  • Potential Benefits in Inequality? The Protective Role of U1 snRNP

    700 Words  | 3 Pages

    snRNPs in cells. U1 snRNP has been found to functions other than splicing, namely in protecting pre-mRNA from premature cleavage and polyadenylation. This protective role may account for its increased levels within cells. In eukaryotic cells, pre-mRNA undergoes extensive post-transcriptional modifications to become mature mRNA. The modifications to pre-mRNA include 5’ end capping, 3’ end cleavage and polyadenylation, and the splicing of introns (Gu and Lima, 2005). The spliceosome is a large

  • Rna Essay : Rna Self Splicing

    2297 Words  | 10 Pages

    Project I: RNA Self-Splicing Kit Fung (Klaus) Chan TA: Christopher Kampmeyer, Henry Sillin Lab Section: 1B T/R 4pm-7:50pm Group Member: Phuong (Nhu) Huynh Group Number: 13 Date Submitted: 4/23/2015 This is my own original work. If any portion of found not to be my own original work, I will accept zero points for this report in addition to whatever the Dean dictates.   Abstract mRNA bears the role of accurately conveying genetic information from DNA into protein (Nature), but there is an extra crucial

  • The Roles Of DNA : The Development Of Complex Proteomes

    1892 Words  | 8 Pages

    The development of complex proteomes without a comparable increase in gene number is due to the different patterns of splicing by the spliceosome machinery. Not only are there mechanics to consider with the spliceosome complex, but there is a kinetic component to splicing as well (Larochelle, 2017). When a DNA sequence is transcribed into a pre-mRNA sequence, it includes exons and introns, which are coding sequences and noncoding sequences respectively. The introns are removed through a two-step

  • Animal 's Ability Foraging Theory

    1663 Words  | 7 Pages

    Miller, and Zentall (2009)was able to show this phenomenon to which they provided pigeons with two alternative keys a 50% discriminative chain, meaning it had two light that represented reinforcement or no reinforcement. A secondly a key that provided food 75% reinforcement on a non-discriminatory chain, meaning that it had a single contingency. what they found was that pigeons would choose the 50% alternative as opposed to the 75% chain despite it providing far more food Stagner and Zentall (2010) at

  • Frankfurt : The Principle Of Alternative Possibilities ( Pap )

    3419 Words  | 14 Pages

    Zhiyuan Li Philosophy 3000 Frankfurt Without Counter-Examples: An Alternative Possibility Harry Frankfurt (1969) argues that the principle of alternative possibilities (PAP): (PAP) a person is morally responsible for what he has done only if he could have done otherwise (829) is false, because there are cases where a person is morally responsible for what she has done even if she could not have done otherwise (835-6). Call such cases Frankfurt-style cases. A lot of literature thereafter has been

  • Congestion Of Congestion Based Fees

    1260 Words  | 6 Pages

    Congestion, particularly in urban centers, has received much attention due to the resulting higher absolute travel times and lower travel time reliability, that lead to higher fuel consumption and significant environmental impacts. In response to the high congestion delays and costs, researchers and policymakers have studied the need and the implications of implementing congestion-based fees in a bid to alleviate congested network links and/or areas, and divert traffic as needed, temporally, spatially