Alternative splicing

CRISPR-CAS9 technique (Fig. 7). We will use a modified plasmid derived from pCAS9 that contains both the CAS9 endonuclease and the Pol III promoter for expressing gRNA. Transformation with two such plasmids, one expressing gRNA prior to the alternating splicing site and one posterior to the NEO marker in the presence of the 6 kb CIN1-L-NEO fragment as donor DNA will result in the replacement of the wild type CIN1 allele with the mutant one (CIN1-L) (Fig. 7). Once verified by RT-PCR and DNA sequencing, Cin1-L

Cryptococcus neoformans (Cn) is a basidiomycete fungus that causes cryptococcosis worldwide in immunodeficiency and healthy individuals. In this pathogen, the elaboration of virulence factors including melanin and the capsule depends on intact intracellular trafficking, an essential cellular process also required for nutrient uptake, ion homeostasis, and receptor recycling. Our previous studies have found that Cn evolves a cryptococcal intersectin (Cin1)-regulated endocytic pathway essential for

the optics-based method offers several advantages such as the kinetic study of splicing and splicing inhibition, study of cis–trans alternative splicing, and rapid measurement of RNA splic-ing. The kinetics of pre-mRNA splicing and the effect of isoginkgetin on the splicing kinetics of the pre-mRNA at the single molecule level were analyzed. Because of their high temporal resolution and the ability to follow the splicing of individual pre-mRNA molecules, the optics-based methods provide the evidences

a specific protein is produced. The first step in this process is called transcription where the enzymes use one of the DNA strands within a gene as a template to produce a messenger RNA or mRNA. The next step in the process is translation. RNA splicing is an important step in creating the mRNA that is involved in protein synthesis in

Project I: RNA Self-Splicing Kit Fung (Klaus) Chan TA: Christopher Kampmeyer, Henry Sillin Lab Section: 1B T/R 4pm-7:50pm Group Member: Phuong (Nhu) Huynh Group Number: 13 Date Submitted: 4/23/2015 This is my own original work. If any portion of found not to be my own original work, I will accept zero points for this report in addition to whatever the Dean dictates.   Abstract mRNA bears the role of accurately conveying genetic information from DNA into protein (Nature), but there is an extra crucial

The development of complex proteomes without a comparable increase in gene number is due to the different patterns of splicing by the spliceosome machinery. Not only are there mechanics to consider with the spliceosome complex, but there is a kinetic component to splicing as well (Larochelle, 2017). When a DNA sequence is transcribed into a pre-mRNA sequence, it includes exons and introns, which are coding sequences and noncoding sequences respectively. The introns are removed through a two-step

snRNPs in cells. U1 snRNP has been found to functions other than splicing, namely in protecting pre-mRNA from premature cleavage and polyadenylation. This protective role may account for its increased levels within cells. In eukaryotic cells, pre-mRNA undergoes extensive post-transcriptional modifications to become mature mRNA. The modifications to pre-mRNA include 5’ end capping, 3’ end cleavage and polyadenylation, and the splicing of introns (Gu and Lima, 2005). The spliceosome is a large

group 2 introns were known to be existed only in mitochondria and chloroplast of plants and fungi, because of the similarity of group 2 splicing mechanism to the nuclear pre-m RNA introns. So it is believed that group 2 introns are the evolutionary ancestors of the nuclear pre-mRNA introns (Sharp 1991). Like nuclear pre-mRNA splicing, even the group 2 splicing start when 2’ OH of a stick out nucleotide attacks the 5’ splice site, this result in the formation of a 2’-5’ bond and a lariat

Design, synthesis and in vitro characterization of an 5′-OH nucleoside carbamate inhibitor of Hint1. Our lab has shown that Hint1 can hydrolyze acyl-adenylates and nucleoside phosphoramidates substrates to generate nucleoside monophospates. We were also the first to design a nucleoside inhibitor of Hint1 by replacing the phosphoramidate backbone in the substrate with a non-hydrolysable carbamate backbone. The designed inhibitor contains guanosine nucleobase instead of an adenosine to avoid off target

ability to provide the same product. * They can gain profit by charging for freight , and storage space The bargaining power of Buyers:- * Price sensitivity * It is strong, as the competition is intense * They have many alternatives suppliers to get the product they desire. * Customer seek FAB (Feature, Advantages and Benefits) Threat of new entrants:- * The threat of new entrants in to the industry is moderate * A significant amount of financial investments