Ampicillin

the transformation; Ampicillin Resistant gene, to sort cells so only the cells having absorbed plasmid will survive; Arabinose, a sugar to aid in the growth of bacteria. The genetic transformation was tested using four samples, each having a different environment. The first sample’s environment consisted of +pGLO, the Luria Bertani broth, and a gene resistant to Ampicillin. The second sample’s environment consisted of +pGLO, the Luria Bertani broth, a gene resistant to Ampicillin, and arabinose. The

Abstract pGLO is a plasmid that contains several genes, araC, gfp,bla, and an ori of replication. E. coli was artificially induced that became a competent bacteria when it took the pGLO DNA, so it had the ability to have ampicillin resistance and fluoresced when arabinose was present. Two tubes with E. coli were labeled to differentiate which tube the pGLO was added to, then through several steps the bacteria was induced to intake the pGLO DNA. At the end, each tube was inoculated

an life form in order to modify the organisms characteristic; the progression of placing genes from one life form to a different is used to assist of a plasmid and the pGLO plasmid codes the gene used for GFP as well as the gene for resistance to ampicillin. It is used to manage the expression of the fluorescent protein; hence, the GFP gene is able to be switched on by adding the sugar arabinose to nutrient medium of the cell, then the bacteria will be able to glow a bright green underneath UV light

an life form in order to modify the organisms characteristic; the progression of placing genes from one life form to a different is used to assist of a plasmid and the pGLO plasmid codes the gene used for GFP as well as the gene for resistance to ampicillin. It is used to manage the expression of the fluorescent protein; hence, the GFP gene is able to be switched on by adding the sugar arabinose to nutrient medium of the cell, then the bacteria will be able to glow a bright green underneath UV light

regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. The ara control

and is set as an example of whether or not the digestion of pUC18 had been successful. Controls 5 and 6 illustrates whether the ligation reactions were successful during the experiment. Control 7 is a negative control, and illustrates whether the ampicillin in the agar solution was working. Reactions 1-3 were set up to locate the optimum ratio of bacteriophage insert DNA to create the most

both the agar plates containing ampicillin and those that did not, it can be concluded that the data provides enough evidence to reject the null hypothesis. There is enough evidence to support the alternative hypothesis stating that there is a correlation between plasmids coding for an antibiotic resistant gene and bacterial growth in ampicillin. When a bacterial solution containing either pUC18 or the lux plasmid is transformed in an agar plate containing ampicillin, only those cells which took in

The Effect of Ampicillin on Bacteria Treated with Plasmid Abstract This laboratory explored the effects plasmid has on the growth of bacteria in the presence of an antibiotic. Four plates were tested: Luria agar +amp -plasmid, Luria agar +amp +plasmid, Luria agar -amp -plasmid, and Luria agar -amp +plasmid. Only two of the plates were treated with plasmid in order to determine the effect it had on the growth of bacteria. The bacteria treated with plasmid was able to grow in the presence of an antibiotic

predicted the E. coli colony transformed with either the PUC18 or the lux plasmid developed an ampicillin resistance. Which made it easier for them to not only survive but also replicate in both the LB agar plates and the LB ampicillin rich agar plate. However the E. coli colony not treated with the plasmids could not survive and colonize in the LB ampicillin rich agar plates. The plate that had no ampicillin in its environment and no plasmid treated E. coli served as a positive control for this experiment

Serratia marcescens Susceptibility to the Antibiotics Streptomycin and Ampicillin Rileigh M. Jones Biological Sciences Student, Cornell University, Ithaca, NY-14853-2401 e-mail: rj246@cornell.edu Abstract-The gram-negative bacteria Serratia marcescens has gained attention in recent years for its tendency to cause nosocomial infections in humans, as well as its development of antibiotic resistance. Antibiotic resistance in a bacterium that is harmful to humans can be concerning as it can result