Analytical Reflective Essay

Method Validation is used to analyze the specific and effective testfor identification of active ingredient. Method validation can be confirmed for their quality, reliability and consistency of analytical results. In this study a simultaneous method for combined two drugs will be discussed.An assay consisted of isocratic elution of Flurbiprofen and Famotidine in Prepacked Column RT 250-4.6 Purospher® STAR RP-18 endcapped (5 µm), using mobile phase composed of acetonitrile, phosphate buffer (pH 3

be performed on field samples that are provided with sufficient volume (generally three times original sample volume). All other blanks, duplicates and control samples will originate in the laboratory and be performed to ensure accuracy of the analytical method, equipment and procedures. These

Co-precipitation Co-precipitation is an important technique widely used for separation and pre-concentration of analytes from various samples [examples]. It is adopted when direct precipitation cannot separate the desired metal ions due to their low concentrations in the sample (Bulut VN et al. 2008). Various mechanisms including surface adsorption, ion exchange, surface precipitation and occlusion are involved in co-precipitation technique [ ]. Metals, at trace levels, react with an organic or inorganic

Studies on chemically deposited Pb(S,Se) thin films J. D. Chaudhari *, R.D. Chaudhari * *, Dr. R. H. Bari + Department of Physics, G. D. M. Arts, K. R. N. Commerce & M. D. Science College, Jamner.Pin – 424 206 Abstract : Thin films of Pb(S,Se) [lead sulfoselenide] have been prepared on glass substrate at 50°C by simple chemical bath deposition technique. The starting materials used for deposition were lead acetate, tri-sodium citrate, sodium sulphite and elemental selenium. Ammonium

Question 1 1.1 In Silica gel chromatograph silica gel is used in chromatography as a stationary phase. In this type of column chromatography, the stationary phase is most often composed of silica gel particles. The silica gel particle sizes can be changed for the achievement of a desired molecular size. Silica gel chromatography separates molecules based on their relative attraction to silica, which is generally greater for more polar molecules than for less polar molecules. Sephadex chromatography

The following results helped obtain the haplogroup that in which the sequence of mtDNA would identify. The PCR reaction worked, and this can be determined by looking at the agarose gel in figure 1. If the PCR reaction was successful, than a band should appear around 550bp. Individual AC displays a band around 550bp, this means the PCR reaction was successful. The band for individual AC, depicts a low concentration of product, because the band faint. After the purification process the concentration

The purpose of this experiment was to determine a series of external standards using Atomic Absorption (AA) and Flame Atomic Emission (FEA) spectroscopy. The principles of Atomic Absorption spectroscopy relies on the ability to distinguish one atom from another with great sensitivity as explained in Quantitative Chemical Analysis by Daniel Harris1. Specifically, this high precise determination can be achieved when specific wavelength of light is generated by a hollow-cathode lamp. The light emitted

High Performance Liquid Chromatography, abbreviated HPLC, is a type of Chromatography that uses liquids to separate mixtures. Unlike other chromatography methods, HPLC uses liquid mixtures for both the mobile and the stationary phases. A syringe is used to inject a small amount of the mixture we’re looking to separate into the HPLC column, and the mobile phase acts as the transporter. The mobile phase contains a carrier liquid that is comprised of all of the dissolved substances that are of interest

Chromatography is the term used to describe all the separation methods based on the distribution of compounds between two separate phases. TLC, an abbreviation for thin layer chromatography, is a chromatographic technique which is used to separate components of a mixture using a thin stationary which has an inert backing, this occurs as one phase is fixed on a plate (stationary phase) and the other phase is mobile and migrates through the stationary phase (mobile phase). During the chromatographic

Last semester’s Honors English lecture was on Sexual Identities in African American Literature. However, the topics often broadened to other areas, one of the most recurring being that of female sexuality and whether literature depicted it as pure and nearly asexual or tainted and virulent. I think this topic is a pressing issue in today’s culture; many states are fine-tuning abortion, rape, and gender-identification laws as I write this. I also saw much attention being paid to this topic in two