Analytical Reflective Essay

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences. Chromatography works on the basis that different molecules have different polarities. By allowing molecules to travel through a polar surface, it is not surprising that molecules will different polarities will travel a different amount. The substance, or stationary phase

In this experiment, thin layer chromatography (TLC) was used to identify and compare polarity of two molecules, caffeine and acetaminophen. Chromatography is defined as the separation of a mixture of chemicals as they flow at different rates over a stationary phase based on their relative polarity. Caffeine, the more polar molecule had a greater affinity for the polar silica gel stationary phase causing it to consistently have a lower retention factor regardless of the mobile phase. This methodology

This experiment demonstrated the separation of pigments based on relative polarity and proved to be a substantial way to separate compounds. The results were much like that of an experiment performed, which separated carbohydrates in a very similar method with the use of paper chromatography (Inome, Y., & Yamamoto, A.). Proper pipetting technique, which is described by John Husler, was also demonstrated in this experiment. The technique was followed as to prevent contamination and deliver the right

Gas-liquid chromatography (GLC) is a process where an unknown organic sample is dissolved in a solvent then vaporised to separate it into its’ components. This is carried out by using two phases; the stationary phase and the mobile phase. The mobile phase is the gas containing the sample and the stationary phase is a liquid absorbed in a solid support. The liquid can be changed depending on the mixture being tested, so the stationary phase is packed in to a long, thin tube called the column (4college

In this experiment, the process of chromatography was taken place in order to identify the different types of pigments present in spinach leaf. This was done by carrying out two types of experiments which are thin layer chromatography (TLC) and paper chromatography. Both of the experiments were done using a similar procedure except that they both used a different stationary phase. Paper chromatography used paper, whilst, TLC used a silica plate. Propanone was used as the extraction solvent for spinach

Sanger settled instead on partition chromatography that had been developed in the1940s by Richard Synge and Archer Martin. This technique is that packed a tube tipped with ground up silica gel, then wetting the gel with water and pipetting in an amino acid solution at the top. Chloroform then inserted to wash the amino acid solution through and added a methyl red dye. The dye formed red bands against to an orange background which helped show up the separated amino acids. First tine Sanger tested

Paper Chromatography and Spectrophotometry Used to Identify Amino Acids and Concentration of Protein in Solution Introduction: Paper chromatography was used to characterize known amino acids in solution with an accordant Rf value. These Rf values were then used to identify a solution with unknown amino acids and observed Rf values. An Rf value is calculated using the following formula:Distance Traveled by solute (cm)Distance Traveled by solvent (cm), where the distance traveled by solute is measured

Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.      Liquid chromatography is an analytical technique that is useful for separating ions or molecules that are dissolved in a liquid phase. If the sample solution is in contact

Isolation and Analysis of Essential Oils using Gas Chromatography Lyndon Justin T. Guzman Institute of Chemistry, University of the Philippines, Diliman, Quezon City Date Performed: February 2; February 4, 2011 Date Submitted: February 18, 2011 Abstract The purpose of this experiment is to isolate the essential oil from eucalyptus leaves as a pure compound; moreover, the components of the essential oil, camphor and limonene, will be then separated using gas chromatography technique, identify the

A Jungian Reading of Beowulf     This essay will propose an alternative means by which to examine the distinctive fusion of historical, mythological, and poetic elements that make up the whole of Beowulf.  Jeffrey Helterman, in a 1968 essay, “Beowulf: The Archetype Enters History,” first recognized Grendel as a representation of the Shadow archetype and identified Grendel’s mother as an archetypal Anima image; I wish to extend the scope of the reading by suggesting that the dragon, too, represents