Bacteriophage

declining. “In the United States, methicillin-resistant S.aureus (MRSA) infections alone account for more deaths than HIV/AIDS and tuberculosis combined” (Abedon). Investing in alternate approaches to medical treatment is necessary and because of that bacteriophage therapy is starting to gain interest. The problem that arises is that

Bacteriophage Lab Report Fady Beshara Montclair State University Biology Lab 112 Section 2 Professor Adams 10/23/16 Bacteriophage lab Bacteriophages were first discovered about 100 years ago by Frederick Twort and Felix D'Herelle. Bacteriophages or commonly known as “phages” are viruses that infect bacterial hosts. Bacteriophages come in multiple shapes and sizes. But a good amount of them are tailed viruses that contain double stranded DNA. The head of the bacteriophages

Bacteriophages are infections that taint microorganisms. That bacteriophages add to destructiveness was initially recommended in 1951, when Freeman found that avirulent strains of Corynebacterium diphtheriae, the causal operator of diphtheria, could be transformed into harmful strains through disease with a bacteriophage. How a bacteriophage can transform a generally innocuous bacterium into a very destructive pathogen turned out to be clear with the investigation of the cholera pathogen Vibrio cholerae

Conditioning of Bacteriophages to Combat Common Diseases through Generational Selection and Isolation In 1928 Scottish biologist Sir Alexander Fleming discovered the remarkable antibiotic penicillin. This drug revolutionized the medical world with its ability to combat infectious disease which had for so long plagued mankind. Though still used today nearly a century later, penicillin and other antibiotic drugs like it are rapidly becoming ineffective at combating diseases. The reason why is like

Before the discovery of antibiotics, research was being conducted on the use of bacteriophages for use in treating infections.16 Among the most abundant type of organism on the planet, bacteriophages present a solution that is species-specific and has less side effects than antibiotics, since eukaryotic cells lack the phage receptors needed for a phage infection.16 Of the two types of bacteriophages, lytic and lysogenic, lytic phages are of great interest due to the fact that they replicate in

In order to isolate bacteriophage specific for Salmonella, we will following procedures below. Firstly, we will inoculate 5 mL tryptic soy broth with Salmonella. Then we touch a colony of Salmonella by using a sterile needle and transfer it to the tube consisting LB broth. After that, we incubate the culture overnight at 37oC. The next day, we will inoculate another tube with 4.5 mL of water sample that we take from poultry farm nearby that we suspect as source of Salmonella contamination on the

Introduction Staphylococcus aureus is a Gram-positive coccal bacterium which is estimated to have colonised 20-30% of the human population.1,2,3 S. aureus is normally found in the anterior nares and mucous membranes of these individuals. For the majority, this is not a problem as these people are colonised, not infected.2,3 However S. aureus is an opportunistic pathogen and if it contaminates a breach in the skin or mucous membranes, it can go on to infect any tissue in the body.3 Infection may

ABSTRACT: Through experimentation new mycobacteriophage genomes were found and added to the scien-tific database. The objective was to find and isolate bacteriophage from soil or water samples, these samples could be taken from anywhere locally. Once the samples were purified through multiple steps, a method of electron microscopy was performed to isolate the viruses’ DNA. The soil sample we found was kept and that phage was infected with a host, m. smegmatis bacterium. Mycobacteriophage or phages

A Planet of Viruses For centuries new viruses have been discovered each and everyday by thousands of different scientists. These viruses affect the lives of almost a billion people. Even though many of these viruses can be harmful to the human body, they have played a phenomenal role in the biological advances over the past century. They move DNA between species, regulate vast populations of organisms, and provide new genetic material for evolution. Viruses influence every single species on earth

and thus must infect other life forms in order to replicate. This characteristic makes them an invaluable tool for the study of molecular cellular processes The T4 phage is a virus that belongs to the bacteriophage family. These viruses utilize bacterial cells as a host for replication. Bacteriophages are bacterial viruses and have been studied for many years. The T4 is a morphologically complex virus with a particularly intriguing shape featuring distinct