Bacteriophage

Before the age 20, I had already been to three different countries and many cities. Which allows me to meet with different people, experience different cultures and encounter different believes. I was born in Mundemba, a small town in the South West region of Cameroon. Cameroon is a located in the western part of central Africa. Life in Cameroon was simple and fun. I spent the first nineteen years of my life there during which I completed high school. After developing and immense love for the sciences

2. Materials and methods 2.1 Bacterial strains, phages and culture conditions The host bacterial strain L. Lactis 7–18, was grown at 30 °C in M17 broth (Oxoid Ltd., Basingstoke, Hampshire, England) supplemented with 0.5% glucose (GM17) (Terzaghi and Sandine, 1975). The thermo-resistant lactococcal virulent phage P1532 used in this study was obtained from the Félix d 'Hérelle Reference Center for Bacterial Viruses (www.phage.ulaval.ca). P1532 phage was amplified on L. lactis 7-18 that was initially

performing enrichment, streak test, titers, harvest assays, spot titers, and preparing of an EM grid to ultimately see the phage that has been chased throughout the semester. This phage was also a myoviridae and named Stubby. INTRODUCTION: A bacteriophage, or phage, is a class of virus, and they are the most abundant life form on earth with approximately 1031 phages worldwide. Phages are parasitic viruses specific to bacteria that can only replicate

We have shown that bacteriophage lambda can be cloned and amplified, ligated and transformed, and inserted successfully into bacterial cloning vector pUC18. The results from the experiments above can be used not only to clone known genes, but also to discover unknown genes that have potential in medicine and gene therapy. Control 4 is a positive control, and is set as an example of whether or not the digestion of pUC18 had been successful. Controls 5 and 6 illustrates whether the ligation reactions

Abstract Due to the rise in antibiotic resistance, bacteriophage research is rising in hopes to be a possible replacement option. Not only do they have the potential to help in the medical realm, but also to help control bacterial populations on food sources. Thus, this study is involved in important research to help isolate and identify a new phage that may be the breakthrough that propels and furthers the research on the high potential of the various applications phages can have in a multitude

popularity in structural biology. Cryo-EM is now beginning to rival X-ray crystallography and NMR techniques to a near atomic details. This essay focuses on how cryo-EM have been implicated in visualizing virus structures with particular emphases on bacteriophage MS2 virion. Introduction: For a mechanistic understanding of macromolecular and biochemical functions and processes of a wide variety of biological structures (i.e. cells, proteins, bacteria and viruses) (Milne et al., 2013) optical devices (i

1. There are many differences between bacteriophages and bacterium. Bacteriophages (also known as phages) are viruses that are obligate intracellular parasites, therefore, need a specific host in order to for this virion to leave its dormant state. The bacterium serves as the host in which the virus inhabits, allowing for it to grow and replicate. According to the case study by Kari Mergenhagen, the virus also obtains and enormous size compared to the bacterium. It has special structures, such as

Materials: The materials that will be used for this experiment are. Overnight to 24-Hour culture of E.coli in TSB. T4 bacteriophage. We will have 8 screw cap tubes that contain 4.5ml of sterile agar that are melted and cooled in 55C water bath. 8 TSA plates were used. 1 tube containing 3ml of TSB was used. We had 2 screw cap tubes, they each contained 9.9ml of TSB. We also had 1ml pipettes with pipette pumps. We also had 1 15ml sterile centrifuge tube. A Bunsen burner. We also had a incubator that

The T-Even bacteriophage is a virus that attacks E. Coli bacteria. The basic function for all viruses is alike. Viruses use this function to hijack cells and inject their own genetic information to reproduce themselves. One cell alone can reproduce hundreds of viruses. This particular virus is good for you, because it kills off the E Coli. infection, which means this is a medical virus that can be used to treat illnesses. The T-Even phage is 90nm wide and 200 nm long, which is relatively big

as the infection is present, rather than decrease rapidly in concentration like antibiotics. The phages stop reproducing once the specific bacteria are destroyed. Phages do not develop secondary resistance, which is very often in antibiotics. Bacteriophages are much more specific than antibiotics. They are harmless not only to the host organism, but also to other beneficial bacteria, such as the gut flora. Phage therapy has few side effects. A small effective dose of phages is effective because they