Chromatography

What is Chromatography? Chromatography is a technique.It is separating mixtures into their components in to analyze.They also use differential affinities.Chromatography is for scientist to study and Analyze,Identify,Purify and Quantify. This can also be a mixture of color .People like scientist,cops, use it for crime and people manufacture plant. Environmental agency,Hospital and Pharmaceutical

1. Two pieces of chromatography paper are trimmed so they are exactly 20.5 cm by 11 cm. The paper is handled by its edges only to avoid contamination from finger oils. 2. The mobile phase is prepared by dissolving a pinch of salt in 50 mL of room temperature distilled water. The salt is stirred until completely dissolved and set aside to use in step 12. 3. Enough mobile phase is transferred to the 400-600 mL beaker so that the liquid level is no more than 5 mm above the bottom of the beaker. The

hopefully will prevent loss of biological activity and denaturation. With ion exchange chromatography comes many advantages over column chromatography such as union between the target protein and functional groups at a very quick rate, exponential growth without difficulty, short processing times, no heating, no extreme pH range, and a multitude of other things (Santos et al. 2012). Ion exchange chromatography in short is ideal due to the low cost, longevity through harsh cleanings, and high

Results When observing the gas chromatography curves, it was evident that there were two pentenes in the unknown mixture, GC_7. This is due to the two peaks on the graph with retention periods (minutes) of 2.802 and 2.883 for the first and second peaks, respectively. The area under the first peak was 36 (m2) and the second was 164 (m2). The compound affiliated with the first peak was 18% of the whole mixture. The compound affiliated with the second peak was 82% of the whole mixture. The

experiment performed, which separated carbohydrates in a very similar method with the use of paper chromatography (Inome, Y., & Yamamoto, A.). Proper pipetting technique, which is described by John Husler, was also demonstrated in this experiment. The technique was followed as to prevent contamination and deliver the right amount of solution each time (John Husler: 1983). The use of paper chromatography to separate plant pigments from spinach leaves worked very well. The pigments were separated into

are. The bigger the dot, the higher the concentration and the farther the dot the more nonpolar it is. A positive test for lipid chromatography appears to have phospholipids, fatty acids and triglycerides in that order on the powdered strip while a negative test appears to not have those three in that order or even have all those three show up. We tested chromatography solvent and placed it on the silica gel strip. Our hypothesis was that the nonpolar molecules in the solvent would move up farther

COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to

CONTENTS S.No. Topic Page No. 1. About the organization 2. Abstract 3. Introduction 4. Thin layer Chromatography • Principle of TLC • Rf value • Applications and Importance of TLC 5. Column Chromatography • Stationary phase • Mobile Phase • Column • Conditions for packing the column 6. High Performance Liquid Chromatography • Principle • Apparatus • Uses of HPLC 7. Paroxetine • Synthesis of Paroxetine. 8. Conclusion. 9. References. ACKNOWLEDGEMENT My eight

were obtained through the use of paper chromatography resulted in four distinct colors. The very goal of this study was to determine what pigments are present and what there absorbance is through the use of paper chromatography and a spectrophotometer. These colors in turn related to pigments that were recognized as chlorophyll a, chlorophyll b, carotene, and xanthophyll. As for the results related to the observations found within the paper chromatography, these four pigments had a distinct relational

Summarized Results To construct a calibration curve, five standard caffeine solutions are prepared and their retention times and peak areas are obtained from the High Performance Liquid Chromatography (HPLC) (Table 1). A graph of peak area versus concentration of caffeine standard solution is plotted (Figure 1). The slope of best fit line and intercept of the calibration curve are 97160384 and -3134906.6 (equation 1 and 2). The standard deviation of slope is 45811392.43 (equation 3). The standard