Coli

I had discovered that my unknown microbe is Escherichia coli (E. coli). After performing various biochemical tests E. coli seemed to fit my microbe the best with only having two misguided biochemical tests that had not fit the data. The tests that were performed included the MRVP test, catalase test, oxidase test, nitrate test, citrate slant test, phenylalanine deaminase test, urea test, SIM test, TSI test, and starch test. These tests helped me discover my microbe along with a few other tests.

E. coli O157:H7 is a gram negative, rod shaped bacteria that is being found in many foodborne and waterborne illnesses. It is also called Hemorrhagic colitis, the acute disease caused by E. coli (EHEC) because it causes abdominal pain, and bloody diarrhea. It generally lives in the intestines of healthy humans and is generally harmless. It is also found in healthy cattle and can contaminate meat during slaughtering, as it produces a toxin that can cause illness. The E.coli O157:H7 is rare variety

bacterial transformation. The plasmids can be altered to include donor DNA thus altering the physiological or physical characteristics of the bacterium. Escherichia coli have been found to be the most effective bacterium in genetic transformation and heterologous expression of human genes or proteins. It was hypothesised that Escherichia coli HB101 K12 could be transformed with a pGLO plasmid to express the genes encoded for green fluorescent protein (GFP), the bla gene for ampicillin resistance and the

Safety Protocols E. coli is a bacterium capable of being transmitted from one person to another. To ensure that the bacteria used in this experiment were not transmitted to other places, items, or organisms, several safety protocols were followed. When streaking bacteria and handling mannitol and xylitol solutions, the experimenter wore an apron, nitrile gloves on both hands and plastic protective goggles. After each set of plates were prepared, the experimenter removed the gloves from both hands

Escherichia coli (or E. coli) is a Gram-negative, rod-shaped, facultative anaerobic bacterium found in the intestinal tract of most species of mammals and birds. E. coli cells contain pili and a flagellum. As a Gram-negative bacterium, they also contain lipopolysaccharides in the outer cell membrane. Refer to Appendix 1 for a labelled diagram of an E. coli cell. The taxonomy of Escherichia coli is as follows: Domain Bacteria Kingdom Eubacteria Phylum Proteobacteria Class Gammaproteobacteria Order

Every year over 96,000 people contract E.Coli, 3,200 of those people are hospitalized for E.Coli, and over 31 people die each year from E.Coli (Food News). E.Coli (otherwise known as Escherichia coli O157:H7) is a disease that is spread in many ways, the most common way being raw and uncooked food in the restaurant being served to the customer. Another way E.Coli is spread is through contact human/animal feces. E.Coli can be prevented, here are some ways to do so; Cook all meats to at least 160 degrees

In this experimental part of the lab, two bacterial suspensions were made by adding 250µl of a transformation solution of calcium chloride, as well as adding and completely dispersing two large colonies of E. coli of approximately 2 mm in diameter, that were collected from the starter plate. Each suspension was labeled, one as +pGLO, and the other as –pGLO. 10µl of p-GLO plasmid was added to the +p-GLO suspension. Both suspensions were incubated for a period of 10 minutes on ice. The bacterial suspensions

nothing so I had to narrow down the search and searched for “bacterial-borne food illnesses.” This led me to most bacterias that caused illnesses from either contaminated food or food that were not properly prepared. On this list I found that Escherichia coli was the suspect, except that the general E.coli bacteria is usually a sorbitol-fermenter and the only exception is one of the strains called “E.coli 0157:H7.” I confirmed this by searching up the sorbitol fermentation and found the sorbitol-MacConkey

possibilities of mutations in a variety of genes and proteins in specific bacteria and ways to combat them. As a model organism, Escherichia coli helps in identifying confounding proteins and enzymes that confer antibiotic resistance. There are specific strains of E. coli that are a common cause of urinary

Transformation Of Escherichia Coli With pGLO Plasmid April 24, 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait, in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid, which carries a gene for ampicillin