DNA Fingerprinting Essay

Exercise #8 DNA Fingerprinting: Identification of DNA Restriction Fragmentation Patterns I. Introduction All humans have in common the coding sequences of their DNA, but, unless you are an identical twin, the non-coding sequences of your DNA are like no other person’s on the planet. The bulk of human DNA does not code for specific genes and is highly repetitive. A British geneticist, Alec Jefferies, developed laboratory techniques in 1984 that became known as DNA fingerprinting. These techniques

ADVANCEMENT IN FORENSIC TECHNOLOGY Forensic science is a field of criminology that utilizes scientific methods to gather and examine evidence in order to prove or disprove a crime. Forensic science involves gathering fingerprints, palm prints, footprints, tooth bite prints, blood, and hair and fiber samples and bunch of other things in order to investigate a crime scene. Ballistics techniques are employed to make out weapons in addition to voice identification methods used to recognize criminals

separation of the fragments was based on the migration of the negatively charged DNA to the positively charged anode. DNA has a uniform mass/charge ratio therefore allowing the molecules to be separated by size within an agarose gel such that the distance travelled is inversely proportional to its molecular weight. The rate of migration is controlled by the following; size of DNA molecule, agarose concentration, DNA conformation, voltage applied presence of ethidium bromide, type of agarose and electrophoresis

2000, evidence from the case was analyzed using current DNA technology’ (Using DNA to Solve Crimes). ‘Then, in February 2001, the DNA sample was matched to an individual who was already serving a five-year sentence for an unrelated 1997 sexual assault of a child. The man has since been convicted of capital murder and aggravated sexual assault.’ In this criminal case, DNA evidence played an important role. A lady was raped, and just because of a DNA sample the criminalist picked up and carefully studied

attended the Indiana University in Bloomington after receiving a Bachelor of Science degree in zoology. Then he received his PhD in zoology in 1950. When he worked at the University of Copenhagen, Watson started his investigation on the structure of DNA and later Watson moved to the University of Cambridge’s Cavendish Laboratory where he met Francis Crick and continued his work. Francis Crick was born in 1916 on June 8th in Northampton, England. He attended Northampton Grammar school and Mill Hill

ABSTRACT DNA is a biological double helix molecule that makes up the genetic component of a person. In the 21st century, there are several applications of DNA’s specific and unique characteristics, such as solving a crime, finding maternity/paternity cases or even research development of genetic diseases. This efficient and accurate system called DNA fingerprinting is a way of organizing the DNA to acquire a person’s identification much like the fingerprint of a person. Unlike the actual fingerprint

They first had to identify whether the body was indeed Tiernan’s or not and two days after the body was found, fingerprinting methods indeed determined that the body was Leanne Tiernan. Despite her body being found 9 months later, her body was still intact enough to gather fingerprints. Fingerprinting proves to be a critical method in forensic cases as no two individuals have been recorded having the same fingerprint, making it an ideal way to identify

Wednesday, July 24, 2013 Total community DNA • Extract DNA from soil – – – – remove cells from soil separate cells from soil lyse cells separate DNA from cells – purify DNA • Extract DNA from soil – Extract DNA from cells in presence of soil • Bead-beating • chemical or enzymatic treatment – Sodium dodecyl sulfate or lysozyme Wednesday, July 24, 2013 DNA purification • Cesium chloride gradient centrifugation • Kits Low density DNA High density Wednesday, July 24, 2013

analysis of DNA using standard Molecular Biology Techniques. During the course of the next three practical classes you shall be performing a number of techniques in order to isolate and manipulate DNA from bacteria. The practicals are spread over three sessions, the techniques that you will perform are indicated below: Practical 1 Isolation of plasmid DNA from three cultures of E.coli using a method known as the alkaline lysis method. Practical 2 (Part A) Digestion of the plasmid DNA that you

structure of DNA. Crick and Wilkins were able to achieve this discovery with the help of an x-ray crystallography of DNA made by Rosalind Franklin, along with contribution from another colleague, Maurice Wilkins. In 1962, Crick, Watson, and Wilkins were awarded the Nobel Prize for this major discovery (Microbeworld, 2006). Franklin had passed away from cancer before the Nobel Prize had been given. This DNA discovery began to develop as early as 1943 when Oswald Avery proved that DNA can carry genetic