DNA ligase

the KLHL3 protein has a N-terminal BTB domain, a C-terminal that has Ketch-like repeats, and a BACK domain, forming a conformation that is a bladed beta propeller structure (1). The protein produced by this gene is part of a complex (E3 ubiquitin ligase), which functions to indicate damaged and additional proteins by labelling these with ubiquitin, which is the tag later recognized by proteasomes for breakdown (4). This labelling is possible with the

The transformation of E. coli using plasmid DNA was a success. The positive control plate had a near lawn of blue colonies growing on the plate. This indicated that the E. coli cells took up the plasmid and became ampicillin resistant. The blue colonies formed because the cells were able to produce β galactoisdase and in presence of X-gal the colonies turned blue. There were light blue colonies formed near the edge of the plate. This could due to the lower concentration of X-gal near the edge of

Discover therapeutic aptamers for VEGF165 and EGFR by high-fidelity pentamer condon set Dehui Kong, Department of Chemistry, University of Georgia Introduction Aptamers are ssDNA or RNA oligonucleotides with very high affinity for their target. They bind to their target with high selectivity and specificity because of their well-defined tertiary structures. Researchers speculate that aptamers have the potential to replace antibodies as high-affinity reagents in medical diagnostics and therapy. SELEX

Nucleic acid is the DNA is transcribed to RNA which is then translated into a protein. These proteins control expression of all characteristics in an organism. The DNA is broken into segments which act as blueprints for various traits of an organism. The best way to analyze DNA fragments is to amplify the fragment and place them into plasmid vectors. Now the question becomes, how exactly is this done? The basic mechanics is that the segment of DNA we need is cut from the full DNA strand. Then the

DNA Ligation and Transformation of E.Coli Badrun nessa Rahman Lab Partner: Briana Tolbert Section 55 March 25th, 2017 Spring 2017 Objective: The purpose of today’s laboratory was to introduce a foreign gene into a cell using recombinant DNA technology by utilizing the techniques of DNA ligation and transformation

All the procedures performed were according to the Biochemical Techniques: Recombinant DNA Laboratory Second Edition (1). Restriction Digestion of Unknown Vector An unknown vector (0.1 µg/µL) was assigned to the group for restriction enzyme digestions. The vector could be either pUWL 500 or pUWL 501.Three enzymes, Pst I, Bgl II, and Eco RV, and their combinations were used to single, double and triple digest the unknown vector. A total of nine tubes of digestion mixtures were made for this experiment

1. The reagents needed to run a restriction digest lambda with the enzyme HindIII will include lambda DNA, 10X restriction Buffer, HindIII and water. 2. The following would be added to an Eppendorf tube: ~5uL lambda DNA(0.5ug/uL) ~5uL 10X restriction buffer ~5uL HindIII ~35uL water This amount would give you a final reaction volume of 50uL. The reaction is mixed and centrifuged to draw the reaction to the bottom of the tube. The reaction was then incubated in a dry bath at 37°C for 1 hour.

purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli), a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way, from cloning and using recombi- nant research, to achieve recombinant DNA. The gene that is the focus of this experiment and is used for copying and expressing is EGFP. EGFP

cloning of plasmid-EZH2 Gene into pBluescript II KS+ plasmid with corresponding Histidine tag for potential analysis Abstract The EZH2 (enhance of zeste homolog2) is an enzyme that in humans is fixed by EZH2 and its supply the information’s about making of enzyme called a histone methyltransferase In this experiment PCR2 was examined whilst the EZH2 contributes to chemical modification. This resulted in repression. The aim of the study was to re-clone the EZH2 gene from 5’ to 3’ into another plasmid

resistance to endogenous and exogenous environmental insults to the genetic material. Multiple DNA repair pathways coordinate the response to such genotoxins and protect our genome [1]. Endogenous insults may be generated as a byproduct of cellular metabolism in the form of reactive oxygen species or during physiological processes such as meiotic recombination, mating-type switching in yeasts, V(D)J recombination and DNA replication-transcription collision [2-5]. Exogenous stresses include hypoxia, radiation