DNA microarray

author’s main analytical questions? Bioinformatics scientist when dealing with DNA microarray data face a challenge of how analysing cluster data in order to discover important knowledge and features (p1). Different clustering methods have been developed to analysis data for gene expression (p1), but none of those approaches introduce a generic solution to overcome the challenge. Most developed applications for DNA microarray are using dendogram and heat-map to visualise the clustering algorithm do not

The emergence of DNA based technologies such as the polymerase chain reaction (PCR) and microarray analysis have been utilized in the rapid detection and identification of many pathogenic bacteria (Mothershed & Whitney, 2006; Versalovic & Lupski, 2002). The expansion of these technologies has significantly enhanced the sensitivity, specificity and the rapid detection of microorganisms (Suo et al., 2010). Microarray technologies have the potential to perform high-throughput

investments became, “NRDO: no research, development only.” Arcadian Microarray Technologies was founded in 2003 by research scientists, some of whom were previously involved in a very successful project called the Human Genome Project. Those scientists helped find links between variations in a person’s genetic code and their predisposition

Tissue microarray Tissue micro arrays (TMAs) was introduced by Kononen et al. in 1998 157. This technique is based on the assembly of separate tissue cores on a paraffin block, which may contain up to 1000 cores. TMA can be used for several purposes, in which the detection of protein is the most common. In this thesis, prognosis/outcome based arrays was applied to evaluate the prognostic value of various proliferation markers. Several advantages and disadvantages are associated with TMA compared

1. Primer Annealing – temperature is lowered so that primers attach to DNA strands 2. Extension – DNA polymerase (e.g. Taq) helps to make new DNA strands. The PCR cycle continues as DNA is copied exponentially. The original PCR methods allowed detection of DNA growth at the end of the process; however the introduction Real Time-PCR (RT-PCR) has allowed DNA amplification progress to be monitored throughout the assay (6). RT-PCR is often confused with reverse-transcriptase PCR (rt-PCR), however rt-PCR

being tested. The only biomedical testing as of now, that should not be restricted from people is DNA Microarrays. According to DNA Microarrays: a Powerful Genomic Tool for Biomedical and Clinical Research, the microarrays can detect diseases and other deformities. "Microarray technologies are now used for comparing other genome features, among individuals and their tissues and cells," (DNA Microarrays) This current use could be used to find new diseases that should not be present. This would allow

According to the genetic information of mRNA, polypeptide is synthesized. 4- mRNA degradation: After translation, the used mRNA is degraded by miRNA’s. 2- (20 pts) a) What are DNA microarrays? In which areas are they used? DNA microarray is a grid that is composed of spots that contains uniform DNA strands. DNA microarrays are used to identify which genes are expressed on a given cell type.

******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted:             a. Genetic testing             b. Body Identification             c. Forensic analysis 3. Name the piece of equipment

However, cancer cells that are too small or have too few organelles will not die. During the second phase, S phase, DNA replication (an identical copy of all the cell’s DNA is made)

1) Describe the structural domains and mechanism of action of ribozymes. How can ribozymes be useful as therapeutic agents? (10 points) RNA molecules that act as enzymes are called ribozymes. They are capable of catalyzing the cleavage of their own RNA or other RNA substrates (Missailidis, 2008). Ribozymes are involved in viroid replication, RNA splicing and protein synthesis. (Clark & Russel, 2005) The structure of ribozymes consist of a catalytic domain and a substrate binding domain. (Ebrahimian