DNA sequencing

Being engaged and intrigued by new information, the love for my coursework has grown every year; however, the opportunity to discover novel information through my research is one of my most intellectually stimulating experiences. Under the guidance of Dr. Nolan Kane, the Professional Investigator of the Cannabis Genomic Research Initiative (CGRI), I have expanded my traditional wet lab work to bioinformatics, hemp breeding, and leading a project I designed to analyze differential RNA expression in

NEXT GENERATION SEQUENCING Introduction DNA sequencing is the method of determining the order of nucleotides in DNA. It includes the method that is used to determine the order of four bases -adenine, thymine, guanine and cytosine in DNA. DNA sequencing has greatly accelerated research and discovery in biological and medical field. The first DNA sequence was obtained using two-dimensional chromatography, in the early 1970s by academic researchers which was laborious. Now DNA sequencing has become easier

Cristillo By Joana Oduro, Melissa Oladokun, Sonia Ottou, Taylor Perry, Sulalith Rajapakse, Meredith Rutledge Table of contents Introduction 3 NGS-based RNA sequencing (RNA-Seq) 4 Chromatin immunoprecipitation sequencing (ChIP-Seq) 7 Paired tumor-normal (T/N) whole-genome sequencing (WGS) 8 References 10 Introduction The relatively recent completion of the Human Genome Project has prompted a change in the approach of a lot of the current

Being engaged and intrigued by new information, my love for my coursework has grown every year; however, the opportunity to discover novel information through my research is one of the most intellectually stimulating experiences. Under the guidance of Dr. Nolan Kane, the Professional Investigator of the Cannabis Genomic Research Initiative (CGRI), I have expanded from traditional bench work to bioinformatics, hemp breeding, and leading and designing my own project analyzing differential RNA expression

scientific research project which was involved in mapping and sequencing human DNA and determining the various aspects of its function. With this incredible expertise to his credit, Dr. Collins in the book ‘The Language of Life’ talks about personalizing medicine by understanding the DNA of each individual. The author starts by narrating some real life incidents of his family members which threw light on the importance of DNA sequencing, then the author goes to talk about various situations and conditions

Figure 1.1: A schematic representation of Sanger sequencing. The Sanger method uses dideoxynucleotides that terminate newly synthesized DNA fragments at specific bases either A, T, C or G. Then, the resulting fragments are resolved by electrophoresis through a denaturing polyacrylamide gel in four parallel lanes, and the DNA sequence can be read (Rosenberg and Pascual, 2014). The initial Sanger sequencing method has been administered to several significant improvements and developed remarkably

Horses are monogstric animals with a relatively small stomach. From the horse’s mouth to their large intestine, their gastrointestinal tract is similar to that of a human’s. However, past the cecum, a horse’s gastrointestinal tract is more similar to a cow’s [3]. A horse’s gastrointestinal tract can be divided into three segments: foregut, midgut, and hindgut [4]. The foregut consists of the esophagus and stomach. Once food has passed through the stomach, it enters the small intestine (midgut): duodenum

What was the human genome sequencing project and was it worth doing? The HGP was a 13-year long project started in 1990 with the objective of determining the entire human euchromatic genome sequence. It was a public funded project and the goal was to complete the project within 15 years. Since its inception, the project had been met with scepticism from scientists and commoners alike. One significant doubt was whether the astounding expenditure of the project would outweigh the potential benefits

Institute, National Institutes of Health 2014a) and to determine the DNA sequence of the complete genome (National Human Genome Research Institute, National Institutes of Health 2014b). The Human genome project enabled researchers to obtain the instructions they needed to gain an understanding of how to build an individual (Genetics Home Reference 2014a). The Human Genome Project made several claims which included to complete sequencing and mapping the genomes of organisms and the ethical, legal and

DNA DTC is a company whose genetic products and services includes research, exome sequencing, and whole genome sequencing, but tests carry a steep price tag. Another company, Genelex is different from the previous companies, and specializes in paternity and pharmacogenomics, which simplified, is the study of individualized effect of drugs based on genetics. Genelex and other companies similar to them could be a threat by repositioning themselves, to combat health based genomic sequencing. A target