Figure 2 represents the agar plates after E. coli bacterial growth. Agar plate (top left) labeled “-DNA” did not contain any bacterial cells with plasmid DNA ,and had cell growth in the form of a lawn. The “-DNA/+AMP” (top right) labeled plate lacked bacterial cells with plasmid DNA, but contained the antibiotic ampicillin. There was no bacterial cell growth observed in this plate. Agar plate (bottom left) labeled “+DNA/+AMP” contained “competent” bacterial cells, as well as ampicillin. For this
Plasmid transformation of E. coli using pVIB Savannah Jacobs April 4th, 2016 BIO 335 Spring 2016 Dr. Koester Abstract Since bacteria are haploid, asexually reproducing organisms it is important for these organisms to be able to accept genetic variability into their genome. A process called transformation, which involves absorbing small segments of DNA from deceased organisms in the natural world, does this. Transformation can also be mimicked in the laboratory using plasmid. Plasmids are small
Background Information: E. coli or Escherichia coli is a prokaryotic cell found the in lower digestive track of mammals and other warm blooded animals. E. coli is an easy bacteria to work with as it doubles quickly and is relatively easy to grow, millions of cells can be grown in several hours. E. coli is an ideal bacteria in the lab because it does not require its temperature to be too hot, too cold, or too precise. A general warm temperature is perfect for this bacteria. E. coli is also easy to care
Transformation of E. Coli With a Transgene and Confirmation of Transgene Cristina Goldy Abstract: In this experiment the goal was to insert the plasmid pBI121 to our E. coli cells. This was accomplished by making our E. coli cells with plasmid competent via heat shock. Our cells were then inoculated onto a kanamycin plate and incubated at 37 degrees Celsius for a day. Our next lab period we preformed a mini prep and extracted our plasmid, through a series of buffers and centrifugation. Once we obtained
I had discovered that my unknown microbe is Escherichia coli (E. coli). After performing various biochemical tests E. coli seemed to fit my microbe the best with only having two misguided biochemical tests that had not fit the data. The tests that were performed included the MRVP test, catalase test, oxidase test, nitrate test, citrate slant test, phenylalanine deaminase test, urea test, SIM test, TSI test, and starch test. These tests helped me discover my microbe along with a few other tests.
Safety Protocols E. coli is a bacterium capable of being transmitted from one person to another. To ensure that the bacteria used in this experiment were not transmitted to other places, items, or organisms, several safety protocols were followed. When streaking bacteria and handling mannitol and xylitol solutions, the experimenter wore an apron, nitrile gloves on both hands and plastic protective goggles. After each set of plates were prepared, the experimenter removed the gloves from both hands
In this experimental part of the lab, two bacterial suspensions were made by adding 250µl of a transformation solution of calcium chloride, as well as adding and completely dispersing two large colonies of E. coli of approximately 2 mm in diameter, that were collected from the starter plate. Each suspension was labeled, one as +pGLO, and the other as –pGLO. 10µl of p-GLO plasmid was added to the +p-GLO suspension. Both suspensions were incubated for a period of 10 minutes on ice. The bacterial suspensions
discovery.” E. coli is one of the most frequent causes of many common bacterial infections, including cholecystitis bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea, and other clinical infections such as neonatal meningitis and pneumonia.” Says the food poison journal, All You Need To Know About E. coli. The bacteria was first called bacterium coli but then was changed to Escherichia coli to honor the scientist that discovered it. There are over 700 serotypes of E. coli
Escherichia coli, or E. coli, is a common bacterium that can be found in diverse environments all over the planet, including the gastrointestinal tracts of animals and humans. Many of these strains of E. Coli are essential mechanisms in the digestive tract, while others are pathogens that can cause complications in urinary and intestinal tracts. (Payne & Sparks) In research, E. Coli is commonly used as a model organism, meaning they are widely studied by scientists for a variety of purposes due to
Most strains of E. coli are not pathogenic; however, some types of E. coli cause painful infections and diseases. Although E. coli resides primarily in the intestinal tract, pathogenic E. coli strains can affect all parts of the human body. The bacteria is mainly spread through contaminated food and water; affecting the victim within 8 days of consuming the organism. The most common type of E. coli-related illnesses is E. coli infection; its symptoms include abdominal cramps, fever and bloody