E. coli

The presentation for our group was to discuss E.coli 1057:H7. Each member of the group was responsible for focusing and explaining their assigned task for the disease. This presentation includes the signs and symptoms, environmental location, transmission, unique characteristics, prevention and treatment. The first part of the presentation was about signs and symptoms of E.coli 1057:H7. Usually signs and symptoms will not occur until about 3-8 days after eating the food that is contaminated with

scientist have used the bacteria Escherichia coli (E. coli) in Synthetic Biology to allow the bacterial species to recognise and respond to light frequencies providing greater knowledge of complex biological systems (Levskaya et al. 2005). E. coli is a suitable chassis to express Green Fluorescent Protein (GFP) as the bacteria’s plasmid helps to establish GFP (Chavshin et al. 2013). The bacterial plasmid is a maltose-binding protein that helps E. coli and GFP fuse together and allows for detection

Escherichia coli, or better known as E. Coli, is one of the most commonly studied single-cell organisms because it is easier to manipulate and it is abundant. Some E. Coli strains can be pathogenic, but most are harmless. They can help benefit digestive health by filling niches in place of more harmful microorganisms. Because it is a diverse group of related microorganisms, it can be difficult to find the boundaries defining the species. Some strains are natural while others are genetically created

Escherich, a German bacteriologist, first discovered E. coli. E. coli is a large, diverse group of bacteria. It continues to be used in labs across the world for experiments and research. Escherichia coli bacteria is most often found in the intestine of people and animals that are healthy. While a few nasty strains of E. coli can cause vomiting, urinary tract infections, respiratory illness, pneumonia, bloody diarrhea, and abdominal cramps, most sets of E. coli are harmless and cause short periods of diarrhea

Thanks for the informative post. Your mention of cluster investigations made me think about the recent E Coli outbreak experienced in several states related to food served at the Chipotle Mexican Grill. According to the CDC (2015), there were two outbreaks, infecting a total of 66 people with a strain of E Coli across 14 states. The CDC, the U.S. Food and Drug Administration, the U.S. Department of Agriculture's Food Safety and Inspection Service, and state and local public health officials in several

side chains on catalytic efficiency, or the efficiency of an enzyme with various substrates. In this experiment, we will determine some of the kinetic properties of alkaline phosphatase (PhoA or ALP), an enzyme that is found in nearly all organisms. E. coli PhoA is a periplasmic enzyme that hydrolyzes phosphate monoesters and has a high (alkaline) pH optimum. For this laboratory, we take advantage of the observation that PhoA hydrolyzes p-nitrophenylphosphate (PNPP, colorless) to give a colored product

The purpose of this lab is to use genetic engineering to transform E. coli bacteria by inserting the plasmid pGLO, and to then see if the bacteria was transformed by using the antibiotic, ampicillin. Background Information: Genetic transformation is the change caused by genes. This transformation includes the insertion of a gene into an organism, changing one of the organism’s traits. There are many other uses for genetic transformation including the altering of plant genes coding for frostbite

The meat and poultry industry is the largest segment of U.S. agriculture. This contributes to the high number of production of meat in the industry. Americans eat meat, eggs and drink milk frequently, but that doesn’t make it good for us. Factory farming is the main issue as to why the way food is processed isn’t good for humans to consume. Animals in factory farming are trained to grow faster than the average animal so that the portions of the food they produce are larger. These animals are sprayed

In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer. A bacterial growth curve illustrates a lag phase, a log phase, a stationary phase, and a death phase

genes (Hartl, 2011). Escherichia coli is a bacterium that has been important to genetic research because it requires little training to handle, is easily maintained, accessible, and inexpensive (Healy & Livingstone, 2010). Another advantage to using E. coli is their quick doubling time and rapid complementation with their operons. Mutant phenotypes reveal themselves on certain medias. All of these reasons have contributed to why E. coli is so well researched. E. coli has an operon that codes for the