Enzyme catalysis

The bombardiers beetle is a small insect that uses a unique form self-defense involving chemical reactions. The beginning of this reaction is in the secretory lobes which produce a highly concentrated mixture of hydrogen peroxide and hydroquinone that is sent to the collecting vesicle. Now, the reaction between hydrogen peroxide and hydroquinone goes like this: the hydrogen peroxide decomposed into water and oxygen; when the oxygen mixes with hydroquinone it produces more water and quinone, a chemical

In our lab, we tested how enzymes and substrates work and react together. An enzyme is a protein that acts as an organic catalyst and lowers the activation energy needed to start a chemical reaction. Each enzyme has only one specific job and is reusable. Enzyme action begins when the substrate fits into the active site of an enzyme, and from there the enzyme can further break down the substrate by putting stress on it, causing the bond to break. In our lab, we used catalase that was found in liver

The results support the hypothesis by revealing that there is a correlation between the higher the concentration of Peroxidase, the faster the Enzyme Activity rate is. The more intense the color is, the faster the Enzyme Activity rate is, and base on the graph, since one minute of the experiment, there is already differences in color intensity between all three groups. Group two with the highest concentration of 3.0mL, during the first minute was at the color intensity of four, while the control

Introduction In this Succinate Dehydrogenase (SDH) project, we were expected to learn the process of cell fractionation, the importance of the Bradford assay, how enzymes work what factors affect them, how to adequately micropipette, how to generate a proper hypothesis and test it, and finally, interpret our results and write a conclusion which answers the central question of this lab: how will the assigned reagent affect SDH activity? The SDH project was completed over a span of three weeks, where

Enzymes lower the activation energy of a reaction by facilitating a proper microenvironment for reaction to take place and by bringing molecules together in the proper orientation. Though hydrogen peroxide does spontaneously react to form water and oxygen

With both the stock substrate and varying enzyme solutions prepared, the Spec20 spectrophotometer was used to investigate the enzymatic activity of β-Galactosidase through an absorbance-based assay. Using LoggerPro software on the computer to analyze the absorption data, the Spec20 was calibrated before each run with 0.5 mL of the tested enzyme concentration at an absorbance of 420 nm. Data collection was then started, instantly followed by the addition of 0.5 mL of the stock 2.5 mM substrate solution

The Effects of Increasing Enzyme Concentration on Enzyme Function Purpose The purpose of this lab is to test for enzyme activity by examining factors that may influence enzymes. Background The reaction taking place is catalase breaking down hydrogen peroxide into oxygen and hydrogen. The enzyme is catalase, the substrate is hydrogen peroxide, and the products are hydrogen and oxygen. The question being tested is when we increase the yeast concentration, what will happen to the rate of the reaction

During this experiment, enzyme activity of catecholase and its relationships with enzyme concentration, pH, temperature, and substrate concentration were tested. Enzymes are proteins that speed up biochemical reactions. By using a spectrophotometer, absorbance rates of the various samples were measured and the enzyme activity for each relationship was observed. While we were testing the effects of enzyme concentration, we found that as the concentration of enzymes in solution increased, the higher

The Effect of low pH on Enzyme Activity Frank Keith Welsh, BIO 102, Fall semester Today I will be providing an experiment on the effects of pH on enzymes. Enzymes are affected by changes in pH. Exceptionally high or low pH values commonly cause in complete loss of activity for most enzymes. Furthermore to include temperature and pH there are other elements, such as ionic strength, that can shake the enzymatic reaction. To each of these both physical and chemical parameters should be considered and

Enzyme inhibitors are molecules that interact with the enzyme in one way or another to stop it from behaving in its natural manner. These have the ability to affect the rate of reaction of SDH. These competitive inhibitors, in our case, Malonate, have a similar shape as the substrate (Succinate) and it binds to the active site of the enzyme (SDH) which prevents the intended substrate from binding and in turn, significantly