Fluorescein

4.2 Materials and Method 4.2.1 Materials The size of the particle should be large enough to allow three proteins coupling together onto one particle. Therefore, Dynal® MyOneTM 1-μm carboxyl particle (Invitrogen®) and Merck® EM1-100/40 particle were used as the carrier particles. If positive results were seen, the work would be expanded to particles in other sizes, materials and manufacturers, such as Allrun® PM3-050 or Dynal® M270 particles. The proteins chosen for coupling were mouse IgG, streptavidin

Fluorescein dye has been used for a very long time and it serves many purposes within the lab and in everyday life. As a product fluorescein appears to be a brownish-orange powder solid that appears to be bright, neon green under a UV lamp. Fluorescein, Yellow No.7 dye, is used as a color addictive to food, medications, and beauty products.2 It is also used as a fluorescent tag for labeling antibodies that will be targeting specific areas of a cell1 or identify and locate life threating thrombotic

These strategies simply target at a model of care for treating DR based on diagnosis rather than an opportunity for preventative eye care and health promotion. Fundus photography, ophthalmoscopy, and fluorescein angiography are the typical tools to diagnose DR and diabetic macular edema [38, 39]. Nonetheless, a widespread range of potential solutions, such as, eyewear innovations, functional tests, groundbreaking eye care treatment, new imaging devices

pKa of around 10-10.5. Since our buffers were at a pH of 8, cytochrome c was unaffected and passed straight through the column. Fluorescein on the other hand has a pKa of about 6.4, so when it was mixed with the buffer a negative charge was induced. The negative charge allowed the fluorescein

The concentration of each of the four protolytic forms of fluorescein at each pH was calculated with Equations 4-7. The total concentration of fluorescein was 14.0±0.1.4 (4) (5) (6) (7) Here Ka is the equilibrium constant of each reaction (reactions 1-3). By comparing the concentration of each of the species at a certain pH value, the pH

Lopez, called fluorescein angiography, which is a method by which ophthalmologists can view the vasculature of the eye. In this technique, a fluorophore is injected into the blood vesicles of the eye, illuminating the vasculature. The benefits of this procedure are that

the fluorescent exclusion dye Propidium Iodide (PI) and Fluorescein Diacetate (FDA), which is a cell permeable esterase substrate. This method is based on the fact that PI is permeable to cells only when there is damage to the cell membrane while FDA is a fluorescein molecule with two acetate groups attached via ester bonds. FDA passes the plasma membrane and live cells then cleaves the acetate groups resulting in a photostable fluorescein molecule that remains in the cell. PI and FDA were purchased

Dry eye is a disorder of the precorneal tear film due to tear deficiency or excessive evaporation which causes damage to the interpalpebral ocular surface and is associated with symptoms of ocular discomfort. The causes of dry eye include defect in the tear film (aqueous layer, mucin layer and lipid layer), lid surface abnormalities and epithelial defects. Around 5% of urban Indian population suffers from Type 2 diabetes mellitus.1,2 Approximately 370 million people across the world are expected

Dyes and Crimes Laboratory Experiment Zainulabidin Adhami AQ8 February 7, 2015 Abstract: In the Dyes and Crimes laboratory experiment, the phosphorescence, fluorescence, and chemiluminescence properties i.e. traits of several chemicals were examined using (UV) Ultraviolet lamp. Observations on color, intensity, duration of glow, etc. were analyzed to determine the traits of the several chemicals. Correspondingly, the author of the unsigned note was determined through ink extraction

Dry eye is a chronic and progressive disease of the ocular surface that affects 30% of the worldwide, aging population.1 According to the 2007 International Dry Eye Workshop (DEWS), dry eye is defined as a multifactorial disease that results in symptoms of discomfort, visual disturbance, and tear film instability.2 Risk factors include, but are not limited to: female gender, age, contact lens wear, systemic disease, use of medications and refractive surgery. Dry eye is an umbrella term encompassing