Gas chromatography

Introduction: Using the analytical methods of Gas Chromatography and Mass Spectrometry, in order to determine the composition of a mixture and its individual pure compounds. This is done by analyzing the mixture of compounds using Gas Chromatography and the individual pure compounds by using Mass Spectrometry. Experimental Procedure: Obtain a screw cap and vial which will be ran in the Gas Chromatograph. Fill vial no more than half full of pre prepared unknown compound which is dissolved in either

In the process of obtaining the products of an elimination reaction between 2-Methyl-2-butanol with a strong acid (sulfuric acid), it was necessary to undergo two fundamental techniques of separation and analysis: distillation and gas chromatography. Distillation is one of the oldest biotechnological techniques utilized by human kind. The history of distillation is very closely tied to the history of the production and consumption of alcohol, although, some of the oldest scientific findings on the

Additionally, it is also important to understand how the percentages of each of the compound is determined. It is mainly possible due to the idea that gas chromatography is utilized. That being said, the equation used in the last lab report is again going to be used here because of the fact that the gas chromatography was used again to get the integrals of the graphs (Lab 2). The equation for that is as follows (Lab 2): (Area of Peak 1)/(Area of Peak 1+Area of Peak 2) x 100 Thus, it is important

Further investigate and determine its identity. By utilizing chromatography, a forensic analyst can identify each material and/or chemical that were meshed together to form the sample under investigation. The identification can be determined once the sample is placed in a liquid or when absorbed on a solid surface, and the separation of the molecules becomes either mobile or stationary. This basic analytical technique is beneficial with classifying drugs that has been collected from a crime scene

Results When observing the gas chromatography curves, it was evident that there were two pentenes in the unknown mixture, GC_7. This is due to the two peaks on the graph with retention periods (minutes) of 2.802 and 2.883 for the first and second peaks, respectively. The area under the first peak was 36 (m2) and the second was 164 (m2). The compound affiliated with the first peak was 18% of the whole mixture. The compound affiliated with the second peak was 82% of the whole mixture. The

Gas-liquid chromatography (GLC) is a process where an unknown organic sample is dissolved in a solvent then vaporised to separate it into its’ components. This is carried out by using two phases; the stationary phase and the mobile phase. The mobile phase is the gas containing the sample and the stationary phase is a liquid absorbed in a solid support. The liquid can be changed depending on the mixture being tested, so the stationary phase is packed in to a long, thin tube called the column (4college

In gas chromatography, the components of the sample, (which can be called solutes or analytes), separate between two phases – a stationary phase that has a large surface area and a gas phase that permeates through the stationary surface (McNair & Miller, 1998). The technique begins when a few microliters of liquid sample are injected into a port and it becomes vaporized to a gas in the chamber. This starts the mobile phase (also known as the carrier gas). Then, an inert carrier gas is fed into the

The extracted organic compounds were analyzed by use of gas chromatography coupled to a mass spectrometer. The sample extracts were injected into a Gas Chromatograph (Agilent 6890) fixed with a capillary column (DB-35MS). The Mass spectrometer used was an Agilent 5973 detector. The temperature of the column was first set at 70 degrees centigrade for 1 minute, then increased at a rate of 10 degrees per minute up to 300 degrees then held at that temperature for 10 minutes. The ion source & the transfer

Gas-Liquid Chromatography Chromatography: Chromatography is a method of analysis in which mixture is partially or completely separated into its components according to relative attraction of the component towards stationery and mobile phase. After the mixture is added to stationery phase, mobile phase is allowed to pass through the stationery phase continuously starting at that point where the sample is added. The mixture is divided between the stationery and mobile phase and move along with the

indefinitely. Gas chromatography and mass spectrometry are the most common methods of chemical analysis. In this report the benefits of gas chromatography will be explored in relation to those of mass spectrometry. It can be concluded that gas chromatography is perceived as a “better” drug detection method than mass spectrometry, however; they are commonly put together as the two techniques complement each other to provide a more through form of drug testing. Gas Chromatography (GC) Gas chromatography