Gram

by the name of Hans Christian Gram. Gram stumbled upon this method while he was examining some lung tissue from patients that had died of pneumonia. While examining this lung tissue Gram discovered that certain stains were more favorable and retained by the bacterial cells. It was only a few years later that Gram produced a staining procedure that he divided into two groups. The two groups divided almost all bacteria into what he called Gram positive (purple) and Gram negative (pink). The Gram’s

find it the very next day. I gram stained my bacteria and I found out I had one gram positive and two gram negatives. I called my gram positive U1 and my gram negative U2 and U3. My gram positive was rods so I was suspected it to be a Bacillus species. I waited for a couple of days for my gram positive to grow old then I endospore stained them to confirm my suspicion. The gram positive was indeed a Bacillus species because of the endospores. At first I thought my gram positive was Bacillus thuringiensis

purpose of this study was to identify the There are a group of tests used specifically to differentiate bacteria in the Enterobacteriaceae family. These Results Table 1 Microscopic Data of Differential Gram Stain Gram Stain (A) Gram-negative Cell Results (B) Gram-positive Cell Results Before Staining Transparent Color Transparent Color After Crystal Violet Stain Purple Color Purple Color After Iodine Stain Purple Color Purple Color After Decolorization with Alcohol Transparent Color

In 2003, 21 Grams was released. This American drama film was directed by Alejandro González Iñárritu and written by Guillermo Arriaga. The writer and director did a wonderful job in bringing each character to life. In the film you can see that the plot wasn’t the main concern, but the characters were. There are three main characters that appear to be very different, living different lives, but have been connected from a tragic car accident. As the story goes on you can see the similarities unfold

In the medical field, certain bacteria need specific antibiotic treatments and to find out what class the bacteria belongs to (whether if it is gram negative or positive) the specimen would have to undergo differential (Gram) staining. Hans Christian Gram was the Danish bacteriologist behind the gram staining technique. His Gram stain technique led to the discovery that Typhus bacillus did not retain the stain. Another procedure like differential staining is called simple staining. The main difference

Gram Positive Bacteria: After picking test tube #2, I inoculated a CNA plate to isolate my Gram positive bacteria. The colistin nalidixic acid in the media inhibits the growth of Gram negative bacteria, the acid affets the outer membrane of the Gram negative so they are unable to reproduce. This test is performed to determine if the Gram positvie bacterium growing on the plate has the ability to break down sheep red bloold cells. After the CNA was incubated in a candle jar, I saw alpha hemolysis

Most bacteria are classified as either gram positive or gram negative. Gram positive is a purple stain and gram negative will appear pink or red. Gram negative can cause many kinds of infections. One type of bacteria is Escherichia which is a rod shaped bacteria. A Gram stain is used in lab setting to identify what type of bacteria is present. A sample is placed in a nutrient media and incubated. This media will encourage bacteria growth present. This process allows for further testing and to identify

discusses the technique of Gram Staining in order to characterize bacteria. Gram-staining is a process in which the cells are immersed in crystal violet, iodine, ethanol, and safranin. Based on bacteria’s cell wall, most common bacteria are either Gram-positive or Gram-negative. The Gram-positive cell wall are composed of multiple layers of peptidoglycan layers, whereas the Gram-negative cell wall has one layer of peptidoglycan. Through the technique of Gram-staining, the Gram-positive cells will turn

specific organism. For example, some gram negative bacteria can produce endotoxins when they die, which would be of concern to a doctor prescribing antibiotics. Identifying a specific bacteria as a source of a disease is the first step in moving towards discovering how it can be transmitted and preventing reoccurrence. Species can be distinguished by morphology and

was done. A Gram Stain was done and the sample was gram positive. A streak plate revealed that the microbe was circular, convex, yellow, buttery and opaque. The next test was the oxidase test. The microbe was oxidase negative. The next test performed was the catalase test. The microbe was catalase positive. The last test performed was a MSA streak plate. The MSA plate turned yellow. This was the final indicator that the unknown microbe 1D was Staphylococcus aureus. Procedure 1 Gram Stain: Purpose: