Gram-negative bacteria

MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed, there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating, identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative

Introduction: This project’s purpose as a whole was to receive an unknown bacteria and figure out what it is; One can figure this out by doing a series of tests. These tests include but are not limited to: gram stains, capsular stains, MacConkey agar plates, SIM, KIA, and UREA tubes, Catalase and Oxidase tests, Methyl Red and Voges-Proskauer test, and many, many more. Although that was just naming some of the tests one can do, what they are and how they’re done are further explained in the methods

be numerous bacteria found there since I see people everyday punching a button to get a cool beverage during breaks in between classes every day. To my surprise, there was only one colony that grew on the agar we had smeared it on. This was very surprising to me because I was sure there would be more bacteria growing on the machine rather than only one colony. The bacteria I was working with occupied a very small amount of space on the agar. The tiny spot, in which I called my bacteria, appeared to

coliforms while the dyes inhibit growth of Gram-positive organisms. As a result, EMB agar allows the inoculation of Escherichia coli, Enterobacter aerogenes, Salmonella typhimurium, and Enterococcus faecalis. To identify E. coli strains on EMB agar, the cultivated bacteria must appear green, black, or have colonies with dark centers (5). While some toothbrushes have bacteria while in the box, the majority of the bacteria is transferred from the bacteria living on the oral cavity. When teeth first

Klebsiella Klebsiella belongs to the tribe Klebsiellae, it is bacteria are Klebsiella Aerogenes and cause urinary tract infection beside causing other health issues. Negative bacteria are rod-shaped and gram negative which belongs to the type of bacteria enterobacteriales. It can be treated by medication but it seems can fight against antibiotic. It caused the high temperature of may exceed 40 degrees, it affects the urinary system. These bacteria live in the colon naturally they do not move, but secrete

1 Week 1 In sterile conditions, 25 grams of sprouts were added to 225 ml of 0.1% peptone. with the help of Stomacher, those two are blended to have a primary dilution of the sample. It acts as the 10-1 dilution, and then aseptically a serial dilution is done upto 10-5 . 100µl of sample from 10-3, 10-4, 10-5 were spread /inoculated on PCA agars. Half of the PCA plates are incubated aerobically , while other half of them are incubated anaerobically. A negative and positive control is prepared by

Enterococcus faecalis, Proteus vulgaris, and Escherichia coli. In each test that I choose it must have at least one bacterium that has different results from 2 other bacteria. Moreover, I performed a motility test to identify if it is motile or not, a Morphology tests which will identify the gram staining and the cell morphology of bacteria and a Catalase to determine the presence of the catalase. Next, I performed a nitrate reduction test to detect if microorganisms are capable of reducing nitrate to

main source of bacteria is this fecal contamination. Fecal matter can enter water sources by runoff from farms or any area that contains many animals. This contamination can also come from insects, rats, or other small animals entering wells or water sources (2). Testing the quality of water, it is often common to test if the water contains coliforms. It is easier to test for coliforms, instead of testing for bacteria that can cause each waterborne disease. Coliforms are gram negative, facultative

and Spirillum has a spiral shape. They all consist of different gram stains such as Cocci can be gram positive in young organisms and might be gram negative in older organisms. Spirillum is gram negative and Bacilli is gram positive. Group B- All of Group B consist of Staphylococcus aureus and Clostridium botulism. Staphylococcus aureus is a small-round shape organism that is gram positive. Clostridium botulism is a rod shape and gram positive. Staphylococcus is mostly related to skin infections such

(Shrestha, 2010) You will need to research the Gram reaction and Acid Fast Reaction for your organism to answer question #3. (Hint: If you know that an organism is G+ or G-, you automatically know its Acid-fast reaction.) (4 pts) 3. You are handed two slides that have been prepared with your organism. One has been properly Gram stained and the other has been properly Acid-Fast stained. a. Describe the appearance of this organism on the slide that has been Gram stained, including morphology. Support your