Gram-positive bacteria

The first unknown test tube P found to be a gram positive bacteria. The biochemical that I performed that were positive were catalase and blood agar. Catalase has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being positive. Also, blood agar plates is useful for cultivating

able to isolate both the gram-negative and gram-positive bacterium. In order to obtain the correct test results and to ensure the right gram mixed unknown is identified there had to be a series of eight biochemical tests performed on the gram-negative bacterium and five biochemical tests performed on the gram-positive bacterium. Introduction The sole purpose behind this conduction was having the ability to understand just how the gram-negative and gram-positive bacteria can be distinguished and

major classes of bacteria that contain peptidoglycan, Gram-positive and Gram- negative. Gram positive bacteria will stain dark purple due to their thick peptidoglycan cell wall, while Gram negative will stain pink due to their thin peptidoglycan cell wall. Many Gram positive and negative bacteria are considered pathogens because they are capable of causing disease in humans. In order to treat properly individuals who are infected with a pathogenic Gram positive or Gram negative bacteria, the identity

Citrobacter freundii. After conducting the Gram stain to ascertain that the unknown bacterium was a Gram negative bacilli, the species were inoculated in multiple biochemical tests. The tests that led to the conclusion of a Gram Negative bacteria were Oxidase, lactose fermentation, MR, VP, Indole, and H2S production. For the oxidase test, there were two categories one was for positive and negative results of the test. The positive result had three different types of bacteria while the negative result was the

Gram-negative and Gram-positive bacteria can be distinguished by conducting biochemical tests. Its growth can be examined on selected mediums containing metabolic inhibitors such as antibiotics. The medias utilized in this project are: media containing the antibiotic vancomycin, Eosin Methylene Blue-lactose (EMB-lactose) agar, and Phenylethyl alcohol (PEA) agar (Holbrook and Leicht 2017). Vancomycin is most effective at inhibiting the growth of Gram-positive cocci (Rotschafer et al. 2005). EMB-lactose

Bacteria have many properties in that some are very harmful, pathogenic, and others are extremely beneficial in many ways to humans and to all types of organisms. Some live within plants, fungi and humans. An example in the human body is Lactobacillus Johnsonii which is a bacteria that helps infants digest milk smoothly (it is found in the gut)( Anwar MA and others 2008). Every type of bacteria is classified according to features that can be studied further for differing properties and what separates

Bacteria can be divided into two types of species, gram positive bacteria and gram negative bacteria. To determine whether a certain type of bacteria was classified tests were conducted by Hans Christian Gram; who discovered the differences within the cell walls of the bacteria. Gram positive bacteria have a thick wall which consists of the protein peptidoglycan. This is opposite to gram negative bacteria which has a thin cell wall, which fold over each other (Wells, 2017). They retain a much thinner

and modes of transmission 4.1 Name one endogenous source of contamination and discuss the modes of transmission from the source to the new host. Endogenous source of infection are already present inside the body in dominant form. It occurs from the bacteria that are already present inside the body. It only invades or attacks the body tissue if circumstances permit. Like if there is any breach in the body tissues. Many bacterial floras are already present inside the body, like those in the gut flora

between Gram positive and Gram negative bacteria. We also used this lab to examine our mixed culture, which had been Gram stained, under the microscope. Theory and background The bacterial cell wall is the outer layer of the cell that aids in structural support and protection from the outside environment. Bacteria can be identified by the structure of their cell wall and classified into two groups known to have different cell wall types. Two of these types are referred to as the Gram positive and Gram

Gram positive bacteria have thick peptidoglycan layer, when alcohol (declorizer) is added it dehydrates peptidoglycan thus causes shrinking and tightening of peptidoglycan. The large crystal violet-iodine complex is not able to penetrate this tightened peptidoglycan layer, and is thus trapped in the cell in Gram positive bacteria. Conversely, the the outer membrane of Gram negative bacteria is degraded and the thinner peptidoglycan layer of Gram negative cells is unable to retain the crystal violet-iodine