Gram Staining Formal Lab Report Miranda King Intro Microbiology Dr. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. This technique uses crystal violet stain, Gram’s Iodine, Ethyl Alcohol, and Safranin. These dyes are used in order to distinguish between the different types of cells. For example
Purpose: The purpose of this lab was to be able to gram stain given cultures and examine their reaction to certain dyes and determine whether the culture is negative or positive, it’s morphology, and arrangement of the cells. By using the Gram staining method, microorganisms can be narrowed down for the identification process as well as the leading to diagnosis Procedure: For this experiment, we were given three gram staining slides as well as a petri dish with five different types of incubated
Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus, Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded, clusters, singly
Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6, 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
question has few symptoms related to this bacterium. The fever, raised his body temperature to 103 ºF and, the respiratory rate of 36 per minute which was caused by pneumonia. Klebsiella pneumoniae is a gram negative bacterium so gram staining of the sputum would be a good place to eliminate all the gram positive bacterium. However it has been reported this test has a
Lab, Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining
of humans, expansion of modern day medicine and improvement of quality of life. In 1884, Hans Christian Gram designed a differential staining technique to identify bacteria that would change the future of microbiology. He give rise to a staining process, known as the Gram stain to differentiate microorganisms into two groups between positive and negative gram staining microorganisms. The Gram stain is essential in a lab technique as it distinguishes the cells based on the physical properties of the
kit & PT kit Biomed diagnostics, Germany n-butanol Sigma, USA Glycerol Acros, USA Ethanol El NASR Pharmaceutical chemicals, Egypt Protenase K enzyme Sigma, USA α-chymotrypsin Amoun Pharmaceutical co, Egypt Catalase enzyme Biodiagnostic, Egypt Gram staining kit Axiom, Egypt H2O2 El NASR Pharmaceutical chemicals, Egypt Bile salts SAS chemicals, India Nutrient broth Oxoid, England Tryptic soy medium Oxoid, England MRS medium Lab M, UK 3.2. Samples collection A total of seven marine sediment samples
“earth. METHODS AND MATERIALS MATERIALS 1 Becker 1 Nutrient agar plate 1 pair plastic gloves 1 Potato agar plate 1 Staining tray 100ml of distilled water 2 MacConkey Agar Plates 3 Blank slides 3 plastic cups 6 pipettes 95% Grams alcohol Bibulous paper Boat & Scale Bunsen Burner & Flint Crystal-violet Grams Iodine Incubator of 35⁰ Celsius (silver) Inoculating loop Microscope Pond water Safranin Soil Tap water Timer