Gram staining

and gram stains. Staining is the process of making bacteria visible to isolate and experiment with. Staining helps to show the bacteria in a color so it is no longer clear by increasing the contrast of the specific organism. Staining is used in the medical field not just to look at bacteria and cultures but also for diagnostic purposes. It can be used to highlight certain tissues and even specific parts within a tissue. There are many different types of staining, including: Acid fast staining ( Ziehl

Staining of bacteria forms the foremost and the most important step in the identification of bacteria. Gram staining: differentiates bacteria into two types Gram positive and Gram negative bacteria Gram positive bacteria can be either cocci or bacilli or vibrios. Gram negative bacteria can be either cocci or bacilli. Motility testing Motility testing is performed by preparing a wet mount and is then observed under the microscope. Biochemical tests The staining will be followed by use of various

Gram Staining Formal Lab Report Miranda King Intro Microbiology Dr. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. This technique uses crystal violet stain, Gram’s Iodine, Ethyl Alcohol, and Safranin. These dyes are used in order to distinguish between the different types of cells. For example

course. It was achieved, by preparing a gram staining procedure, to help us identify the morphology of the bacteria, and to prepare a various of different test, such as the starch test, oxidase, urease, citrate, MSA, Nitrate Reduction and MP/VP to help us identify if the bacteria is able to produce the specific catabolism that it being testes for. Procedure: Gram Staining             Gram Staining is a procedure used to distinguish between gram-positive and gram-negative bacteria, where one of the main

Tiffany O’Connor 9/2017 Gram-positive and Gram-negative stain Purpose: Staining is done to differentiate between two varieties of bacteria, gram-positive and gram negative. Theory and Background: Gram-positive and Gram-negative represents whether a cell wall retains the primary dye color in the staining process after decolorization. The difference in the structure of the cell wall determine if this happens. In Gram-positive cells the walls are much thicker than Gram-negative. Gram-positive cell walls

Gram Stain In 1884, a Danish pathologist, Christian Gram, discovered a method of staining bacteria with pararosaniline dyes. By using two different dyes, he discovered that bacteria fall into two different groups. The first group, gram positive, retains the primary color, crystal violet. The second group, gram negative, when washed in a decolorizing solution, becomes colorless and takes on the color of the counterstain. The reaction of the Gram-stain in a bacteria is determined by the biochemical

Purpose: The purpose of this lab was to be able to gram stain given cultures and examine their reaction to certain dyes and determine whether the culture is negative or positive, it’s morphology, and arrangement of the cells. By using the Gram staining method, microorganisms can be narrowed down for the identification process as well as the leading to diagnosis Procedure: For this experiment, we were given three gram staining slides as well as a petri dish with five different types of incubated

identical daughter cells each with identical DNA to the parent cell. Gram staining, also known as Gram's method, is the most important and universally used staining technique in the microbiology and bacteriology laboratory. It is almost always the first test performed in the identification of bacteria (Xu, George).The

celled prokaryote microorganisms, rapid mutations causes bacteria can be found just about anywhere.Gram staining helps to classify two groups of bacteria, Gram-positive and Gram-negative. Gram-positive bacteria's cell wall has a stronger attraction for crystal violet, because of more peptidoglycan. Gram-negative is a group of bacteria that doesn’t retain the crystal violet stain used in the Gram staining method, making positive identification possible. These are the many characteristics used to describe

Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus, Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded, clusters, singly