Gram staining

Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria

why it is essential that gram stain and microscopic examinations are the first step in identifying pathogenic bacteria. Gram stains are an important procedure because it can determine the stain of the cell wall of bacteria sample. It also allows laboratory technicians to classify the bacteria in to either gram positive or negative as well as allowing them to analyse and study the morphology and arrangement of bacteria. Whilst determining the bacteria type and type of gram stain, the test also allows

The results that I observed by Gram Staining was Gram Negative rod (bacillus). Then, I performed a Negative Stain in order to further more validate that I had bacillus, since Negative Staining would provide an accurate determination of the shape of the microbe by staining the background of the microbe. In observation of my unknown using Negative Staining; I knew that I had a microbe with bacillus. Day#2: 　　I did a Fermentation Test where I inoculated

oxidase test. This test is for the gram negative bacteria only; unknown species A. Using a plastic inoculating loop pick up the gram negative bacteria and smear it onto an oxidase Drislide. If the spot on the paper turns purple within 20 seconds, the test is positive. If the spot on the paper does not change color, the test is negative.1 Lastly is the Amylase test. This test is for only gram positive bacteria; unknown species B. Using a starch agar plate inoculate gram positive bacteria onto it. Make

Lab, Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining

INTRODUCTION Unknown samples were distributed to students for the purpose of identifying the sample. Multiple biochemical tests, like phenylethyl alcohol agar and decarboxylase test, were conducted on the unknown sample. The goal of the biochemical tests was to practice using the techniques learned in the microbiology lab and gain experience performing the different tests. Further, the tests allowed for the identification of an unknown from the recorded results. These tests were conducted in order

of humans, expansion of modern day medicine and improvement of quality of life. In 1884, Hans Christian Gram designed a differential staining technique to identify bacteria that would change the future of microbiology. He give rise to a staining process, known as the Gram stain to differentiate microorganisms into two groups between positive and negative gram staining microorganisms. The Gram stain is essential in a lab technique as it distinguishes the cells based on the physical properties of the

significant in a microbiology lab but also in the medical, manufacturing, and pharmaceutical fields. The first test used in the identification of unknown bacteria numbers 3 and 4 was the Gram stain, which was used to differentiate between gram-positive and gram-negative bacteria based on their different cell wall elements. The Gram stain procedure distinguishes between

kit & PT kit Biomed diagnostics, Germany n-butanol Sigma, USA Glycerol Acros, USA Ethanol El NASR Pharmaceutical chemicals, Egypt Protenase K enzyme Sigma, USA α-chymotrypsin Amoun Pharmaceutical co, Egypt Catalase enzyme Biodiagnostic, Egypt Gram staining kit Axiom, Egypt H2O2 El NASR Pharmaceutical chemicals, Egypt Bile salts SAS chemicals, India Nutrient broth Oxoid, England Tryptic soy medium Oxoid, England MRS medium Lab M, UK 3.2. Samples collection A total of seven marine sediment samples

Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6, 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide