Gram staining

The Ames test is an initial screening assay that can be used to determine the likelihood that the chemical being tested will be mutagenic. This test works by using different strains of the bacterium Salmonella typhimurium that are carrying a mutant gene making it incapable of producing the amino acid histidine from the ingredients in the culture medium. These mutations have the ability to be reversed and restore the functional sequence to the gene; which will allow the bacteria to synthesize histidine

Gram Stain: The Gram Stain test was performed on our unknown bacteria and the results were negative. Meaning that the cell wall of the bacteria did not retain the purple dye, therefore it classified it as Gram-negative (-). After observing the slide under the microscope, (Figure 1 below) our bacteria’s morphology was identified as rod-shaped/bacillus. Figure 1: (Klebsiella pneumoniae mixture, Under 100X oil immersion) Oxidase Test: To test for the presence of cytochrome oxidase enzyme, the oxidase

10, were Proteus mirabilis and Staphylococcus saprophyticus. Proteus mirabilis being the rod shaped, gram-negative bacteria that grew were the small, shiny, and round with a convex elevation, and Staphylococcus saprophyticus being the cocci shaped, gram-positive bacteria that grew a creamy, off white color and round. Proteus mirabilis comes from the Enterobacteriaceae family, which consist of gram-negative bacteria some being harmless, while the majority ae considered as pathogens. “Within this family

The purpose of the bacterial unknown independent study experiment completed throughout the course of this lab was to determine the identity of an unknown bacterial species. The unknown bacteria sample was chosen from numerous samples provided by the instructor. The starting unknown sample, unknown #15 was a mixed bacterial culture and a broad approach taken to identify the sample. Various biochemical tests were completed to identify the bacterial species along with the use of databases such as Gideon

This microbe was identified from colonies appearing on both plates. Gram stain results: gram negative rods. Acid from glucose: positive. Gas from glucose: positive. Lactose fermentation: negative. Sucrose fermentation: positive. DISCUSSION - Carbohydrate testing utilizes the different ways that

Introduction Termites are small insects. They are very social animals. They have three basic groups in their society. Like any other animal communication is very important to termites. Termites use chemicals known as pheromones for communicating. The solvents in the ink of Papermate and Bic ballpoint pens have been discovered to be similar to the pheromones termites use for communication. The purpose for doing this lab is to have a better understanding of termites and communication. Hypothesis:

The purpose of this lab was to save a little gummy worm named Sam from drowning. In addition, one of the labs key terms was a hypothesis which is a supposition or proposed explanation made on the basis of limited evidence as a starting point for further investigation. Furthermore, we also learned that an independent variable is a factor that is changed during the experiment. On the other hand, the dependent variable is the factor that is observed or measured during the experiment. To begin with,

from colonies, so that organisms can be identified. I inoculated a MacConckey to help me identify my Gram negative organism and observed that the organism was a non-lactose fermenter, then I performed a Citrase test which was negative. The last test I performed was a TSI, the butt was positive for hydrogen sulfide production and fermentation of glucose. All 3 results helped me conclude that my Gram negative organism was Proteus vulgaris. P vulgaris inhabits the human intestinal tract of human and

genus and species of these morphologically identical unknown bacteria. The purpose of this lab was to identify a mixed pure culture of two unknown bacteria, by applying various differential tests that were learned in the lab thus far. Based upon the Gram-stain reaction and the reactions that occur on the media that they grow on, it is then classified into categories of genus. After each unknown culture has been successfully isolated specific biochemical tests were performed and their metabolic pathways

There was a mix up with placement of the Plasmid containing Bacteria (experiment’s culture) and control bacteria. The experiment’s culture was place in the jar with no ampicillin. As a result, bacteria with plasmid grew as well as bacteria without plasmid. However, it is possible to identify the transformed culture because of the blue marker. On the other hand, the control cells do not have the plasmids with ampicillin resistance and blue marker so the population of bacteria cells are fewer in the