Gram staining

particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size

June 2, 2015 Gram Stain Laboratory Report Purpose: To understand how the use of differential stains can help us identify cell morphology. Also to identify the presence of two different bacteria in our mixed sample by identifying differences in color, shape and grouping of eubacteria. Theory and Background: Gram stain is the most important and “commonly used differential stain in microbiology” (1). Developed by Hans Christian Gram in 1884, while searching for a way to

Bio Pre Proposal I. OBJECTIVE: To have the ability to identify gram positive and gram negative cells and understand how the staining affects them. II. HYPOTHESIS: if a smear of both Escherichia coli and Staphylococcus epidermidis are stained, then gram negative and gram positive stains should result respectively and be identifiable under the microscope due to the structure of the cell walls. III. PROCEDURE: 1. Obtain a blank, sterile microscope slide. Using an inoculating loop add one drop of distilled

about the chemistry of a specimen a method called, staining is used to increase its differences. Stains/dyes are a salt that colors the ion it penetrates. There are two types of colors retrieved when staining; in a basic stain the color appears in the positively charged ion, while in an acidic stain the color is in the negatively charged ion. Examples of basic dyes include: methylene blue, crystal violet, and safranin. Generally, if a staining procedure only uses one

work with an unknown organism and understand the isolation of an environmental sample. It started out as a swab on a surface of the student’s choice and placed on a slant agar to grow. After a few weeks we were able to see growth on the agar. Simple staining is one quickest methods for testing the presence, determining the shape, or numbers of bacteria in sample. The bacterial smear is stained with a single dye and produces a distinct

tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart, I proceeded to inoculate a Starch plate. In week 2, I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative due to the dark color. Next, I inoculated Simmons Citrate Agar slants. By week 3 I was able to analyze the Citrate Agar, which tested negative

bacteria. After performing Gram staining and deciphering if the unknown was gram positive or negative the lab officer was then able to proceed to the next step of identification. The gram positive unknown’s reaction to the catalase test informs the tester of the Genus theyre working with. This indicates which tests to perform next. The MacConkey agar is a selective medium that only allows the growth of gram positive bacteria confirming the results received from the gram staining procedure. The NaCl growth

infections, it can cause serious infections such as bloodstream infection, pneumonia or bone infections. Though it can cause infections it is part of the normal human flora it is mostly found on the skin or in nasal cavities. It is a facultative anaerobic gram positive cocci, it is usually in pairs or clusters. The bacterium is also catalase positive, oxidase

After isolating the DNA pellet and gram staining the sample, it could be concluded that the DNA found in the water sample was gram-negative. This is because the crystal violet stain did not remain on the sample and the sample showed red. Gram-negative means the sample has thin walls, making it easier to break the wall and get access to the DNA. The sample was stratified squamous and had a strep bacilli growth pattern. This is essential to a thorough lab analysis of water contaminants because it helps

Peptidoglycan is also present in large amounts in the gram positive microbes. While the gram negative microbes also contain peptidoglycan it is only present in a small amount. Other microorganisms contain other components in their cell walls that prevent them from being gram stained. There are four important ingredients in the gram stain technique. These are the 2 stains crystal violet and safranin along with Gram’s iodine and 95% ethyl alcohol