Growth medium

pH and any other specific conditions were provided. The most basic and common medium of growth on the nutrient broth, which contains water, beef extract and peptone. Although most bacteria will grow in this medium, some bacteria are fastidious and require special growth supplements such as blood or sugar. Further in mixed populations where it would be difficult to isolate the minority type of organism in a simple medium. Therefore, media can be divided into different types depending on the chemical

Microbial Unknowns Objective: In the past couple of weeks, our class received a single broth that contained two species of bacterium. One bacterium was gram positive and one bacterium was gram negative. We were required to use our techniques and knowledge we learned in class to separate and identify the two species we were given in our broths. Methodology: Day One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of

unicellular. Bacteria are the most abundant microorganism. They are among the earliest forms of life that appeared on earth billions of years ago. They can grow in different temperatures and many exist naturally in our human flora, such as staphylococci. Growth within different temperatures can range from 0 degrees Celsius such as psychrotrophs, to hyperthermophiles who grown best above 70 degrees Celsius (Ritchey, Exercise 16). Bacteria have many shapes, size, and multicell arrangements. The main bacteria

For this lab, the direct viable count method using TSA medium was used to find the Colony forming unit due to its simplicity. Unlike other methods, direct viable count only measures the number of colonies that were able to form on the medium that was used. After the number of colonies were recorded, the CFU was found based on the total number of colonies, dilution factor and the volume of liquid

production and high demands in the market. As elaborated by Singh, Uma, Selvarajan and Karihaloo (2011), there are five steps in banana shoot-tip culture namely selecting of sucker, sterilizing of instruments, medium and environment, introducing the explant to Murashigee Skoog (MS) medium, sub-culturing the callus, and lastly is weaning and hardening of plantlets. (refer to Figure 1 in Appendix 1) The first step in banana shoot-tip tissue culture technique is selection of healthy sucker. During this

that can affect the growth of a bacteria. These influences include the presence of oxygen, pH, the accessibility of water, and temperature. The abundance or lack thereof for each of these factors can determine whether a bacterial cell is able to propagate or if it will ultimately die. Additionally, the intensity of these factors can also determine the amount of growth seen. In order to observe this relationship one of the influential factors was chosen and the rate of growth was observed. Temperature

How does the addition of pGLO plasmid to a solution containing E. coli bacteria affect the growth and characteristics of the bacteria? Genetic transformation is the incorporation of foreign DNA into an organism to potentially change the organism’s trait. Plasmids are small circular DNA that replicate separately from the bacterial chromosome. In nature, these plasmids can be transferred between bacteria allowing for the sharing of beneficial genes. Due to this characteristic, plasmids allow for genetic

2015 2:30 p.m. to 5:30 p.m. Introduction The purpose of the lab was to use selective, differential and enriched microbiological media’s to distinguish the growth characteristics of microorganisms. A selective media was used in a mixed culture to inhibit the growth of certain microbes, but not affect the desired organisms’ growth by the use of chemicals (Duxbury, 2015). The differential media was used to differentiate between closely related organisms by using certain dyes or chemicals (Possé

multicellular organisms growing in the absence of other species or types. A method in which microbes are cultured by letting them reproduce in the predominated culture media under controlled laboratory conditions, its abundance in the medium being checked before contamination and after contamination. It is one of the primary diagnostic method of microbiology and used as a tool to determine by letting the agent multiply in a

pouring plates. These can then be stored for up to a month at 4˚C, ensuring to dry in a flow-bench before use. M9 minimal medium glucose plates: 15g Agar should be dissolved in 800ml water (deionised) before being autoclaved. This should be cooled to 50˚C, before adding 200ml 5xM9 salts as well as 1ml of 1M MgSO4, 100micrograms of 1M CaCl2 and 1ml of 1mg/ml VitB1. 2xTY medium: 16g bacto-tryptone should be dissolved, as well as 10g yeast extract and 5g NaCl all within 1L of water (deionised), before