HEK cell

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  • Synthesis And Function Of The Dopamine Transporter

    2061 Words  | 9 Pages

    staining, a thorough understanding of proper sterile techniques is needed. Sterility refers to cell culturing experiments without any contamination, therefore all of the experiment was performed under the hood. No discoloration signified no contamination. The entire experiment proved successful upon identifying the YFP dopamine followed by colocalization and concluding with a release of calcium in the cell. Introduction Structure and function of the dopamine transporter The dopamine active

  • Phosphorylation At S13 And S114 Case Study 1

    825 Words  | 4 Pages

    Validating the candidate kinases phosphorylating at S13/S16 and S116 Kinase gain of function (GOF) analysis: The hit cDNAs of kinases will be co expressed along with cDNAs Htt exon 1, Htt 171 and Htt 586 containing 16Q-23Q in HEK 293 cells and the Phosho S13/s16 and S116 Htt will be examined using the appropriate antibody, Phos –tag SDS-PAGE method (Bustamante et al., 2015) also method of IP of Htt and mass spectrometry. Kinases will also be co expressed with appropriate S to A variants of the above

  • The Relationship Between Grks And Arrestins

    748 Words  | 3 Pages

    phosphorylation of the S869-V893 region of mGluR1a when expressed in HEK cells (Iacovelli 2003, Sallese 2000, Mundell 2003). These agonist-induced internalization processes are -arrestin 1/2 and dynamin-dependent (Mundell 2001, 2002, 2003). -arrestin 1 appears to be important in mGluR1 endocytosis; however, the agonist-stimulated internalization of mGluR1a is observed only when -arrestin 1 is co-expressed with either GRK2 or GRK5 in HEK cells. GRK2, GRK5, -arrestin 1, or -arrestin2 individually has no

  • Specific Sites On Sec24c / D Regulate Copii Trafficking And Mediate Protein Proteins Interactions Essay

    1632 Words  | 7 Pages

    Examine the Interplay between O-GlcNAcylation and phosphorylation on Sec24C/D in cell cycle progression and vesicle trafficking. Aim1: Determine how O-GlcNAcylation of Sec24C/D affects COPII vesicle secretion under normal and ER stress conditions. Research design: I previously identified nine O-GlcNAc sites on Sec24C. This was accomplished by expressing myc-6xHis tagged Sec24C in human embryonic kidney 293T cells treated with Thiamet-G, an OGA inhibitor, and glucosamine to increase O-GlcNAc occupancy

  • Abnormal Intracellular Zinc Metabolism Caused By Disruption Of The Trpl1 Tmem163 Protein Interaction

    948 Words  | 4 Pages

    levels of specific trace metals have been suggested to contribute to the abnormal cellular phenotype, as well as the progressive cell degeneration associated with TRPML1 dysfunction4. The permeability of Zn2+ to TRPML1 suggests that this ion channel may play a role in Zn2+ regulation. A recent investigation of cytoplasmic Zn2+ accumulation in TRPML1 dysfunctional cells suggests the possibility that this protein may normally function to transfer Zn2+ from the lysosome to the cytoplasm5. Other studies

  • Autophagosomal Pool Flow

    960 Words  | 4 Pages

    Rluc-LC3-assay can be used to estimate autophagic flux at a single time point by defining the luciferase activities in cell extracts. Moreover, the use of a live cell luciferase substrate makes it possible to monitor changes in autophagic activity in live cells in real time. This method has been successfully used to identify positive and negative regulators of autophagy from cells treated with microRNA, siRNA and small molecule libraries.242- Farkas T, Hoyer-Hansen M, Jaattela M. Identification of

  • Boyce Lab Report

    411 Words  | 2 Pages

    challenging due to the low-affinity, sub-stoichiometric and transient nature of the modification. Using a synthetic O-GlcNAc analog containing a diazirine photocrosslinking moiety (GlcNDAz), we are able to label native O-GlcNAcylated substrates in live cells and crosslink them to nearby glycosylation-mediated binding partners upon UV treatment 13. In experiments performed by Abhisheck Chhetri, Tim Smith and Thomas Meister, the Boyce lab showed that endogenous and recombinant Myc-6xHis-tagged Sec24C exhibit

  • Dysbindin : A Study

    886 Words  | 4 Pages

    and Student’s t-test was used for post-hoc analysis between vector and Dys-siRNA. (E) Quantification for YFP-Drp1 puncta on mitochondria; paired t-test was used to compare the same cells before and after stimulation. (F) Quantification for YFP-Drp1 puncta on mitochondria; paired t-test was used to compare the same cells in the vector and the Dys-siRNA group for before and

  • Mechanisms Of Ebv Latent Membrane Protein Trafficking Essay

    1490 Words  | 6 Pages

    protein 1 (LMP1) is expressed in most EBV-associated cancers and it is well established that LMP1 is a major viral oncogene. Expression of LMP1 alone is sufficient to transform cells and recombinant EBV lacking LMP1 is incapable of immortalizing B-cells in vitro. Moreover, transgenic mice expressing LMP1 behind a B-cell specific promoter develop lymphomas. Exosomes are a population of small (40-150 nm) endocytically-derived extracellular vesicles produced from inward budding events on the limiting

  • Module6LabAnswerSheet RANKOW Essay

    1184 Words  | 5 Pages

    becomes tightly wound and the genes are inaccessible. b. What happens to the amount of GFP mRNA? It reduces, there is almost none left. c. What happens to the amount of green fluorescent protein? It also reduces. d. What happens to the appearance of the cell? It becomes faded with a faint green color. 3. Click on the Labels button near the control knob to reveal the following three links that reveal information when clicked. Summarize the function of each: a. a. histone – these are a type of protein that

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