High Performance Work Essay

of the main differences between HPLC and UPLC can be seen in the table below: Attribute HPLC UPLC Pressure 6000 Psi 100,000 Psi Particle Size 5μm 1.7μm Flow Rate Millilitres per minute Microlitres per minute Max Resolution Relatively low Relatively high The lower particle size is the true reason for UPLC increased flow rate and resolution. This can be shown mathematically using Van Deemter's equation: H = A + B/µ + Cµ. H being the plate height and µ being the particle size. The A constant remains

developing sensitive, simple, and rapid method for determination of DXM has been become an important target in control pharmaceutical dosage form. Therefore wide range of analytical methods for quantitation of DXM have been developed such as, high performance liquid Chromatography(HPLC) (Abdel-Moety, Al-Deeb, and Khattab 1995, Tedesco et al. 2013, Eichhold et al. 1997, Hoskins, Shenfield, and Gross 1997) (Abdel-Moety, Al-Deeb, and Khattab 1995, Tedesco et al. 2013, Eichhold et al. 1997, Hoskins, Shenfield

A simple and rapid reversed phase-high performance liquid chromatographic (RP-HPLC) method was proposed for simultaneous determination of imipramine hydrochloride and diazepam in pharmaceutical formulations. Elution was done utilizing methanol: water: 0.1%m sodium acetate (30:50:20 v/v/v) mobile phase with Chromosil C18 column in isocratic mode, at a flow rate of 1.0 ml/minute and detection wavelength of 243 nm. The measured retention times for imipramine hydrochloride and diazepam were 3.33±0.02

High Performance Liquid Chromatography, abbreviated HPLC, is a type of Chromatography that uses liquids to separate mixtures. Unlike other chromatography methods, HPLC uses liquid mixtures for both the mobile and the stationary phases. A syringe is used to inject a small amount of the mixture we’re looking to separate into the HPLC column, and the mobile phase acts as the transporter. The mobile phase contains a carrier liquid that is comprised of all of the dissolved substances that are of interest

spectroscopy, I.Rspectroscopy ,NMR spectroscopy, Flourimetry.  Chromatographic methods : Where we study affinity or artition co-efficient differences of drugs and pharmaceuticals. Eg:TLC(Thin layer chromatography),HPLC(High pressure liquid chromatography), GC(Gas chromatography), HPTLC(high pressure thin layer chromatography), UPLC(Ultra pressure liquid chromatography). Chromatography Column

lipid in the membrane they are transported and hence the degradation takes place for other activities to begin. This lipid can be detected by instrumental techniques HPLC and TLC. The related diseases and functions are determined. Various experimental work is being performed on this lipid. And being a helpful lipid it is used in determining various disorders caused in human body. INTRODUCTION: Glycoglycerolipids exists in plants, prokaryotes, eukaryotes but extensively found in marine algae (1,2,3).

and benzoic acid are common additives to beverages for the stimulation effect and as a preservative, respectively. To simultaneously determine the amount of each of these chemicals, a method combining UV/Visible spectroscopy and reversed-phase high performance liquid chromatography was introduced. The experimentally determined concentrations at the 95% confidence interval of caffeine and benzoic acid in Mountain Dew were 149 +/- 5 ppm and 308 +/- 6 ppm, respectively. The method showed separation of

time.      Conventional Liquid Chromatography is most commonly used in preparative scale work to purify and isolate some components of a mixture. It’s also used in ultra trace separations where small disposable columns are used once and then discarded. Analytical separations of solutions for detection or quantification typically use more sophisticated high-pressure liquid chromatography instruments.      In liquid chromatography, the separator is

Introduction Although paracetamol was used clinically for the first time by Joseph von Mering in 1883 and it has been commercialized since 1953, its mechanism has not been discovered yet. However, paracetamol is the most used drug to fight fever and pain currently due to its antipyretic and analgesic properties. The aim of the experiment is the production of paracetamol by formation of an amide bound between the amine group of p-aminophenol and the carbonyl group of acetic anhydride. Furthermore

yields high performance and high speed compared with traditional column chromatography because of the forcibly pumped mobile phase. Recently, ultrafast analysis using a high-pressure-resistant apparatus has been attracting attention. UHPLC (Ultra High Performance LC) is becoming established as an abbreviation for this ultrafast LC method. In 1941 Martin and Synge, described the discovery of liquid-liquid partition chromatography and also laid the foundation of Gas liquid chromatography and High performance