High Performance Work Essay

and development of sub 2μm particles to capitalize on advantages of smaller size particles (Jerkovich et al., 2003; Wu et al., 2001). Nonporous 1.5μm particles with high efficiency columns are commercially available, but they have some disadvantages of low surface area, leading to poor loading capacity and retention. To withstand high pressures, UPLC must use a novel porous particle to maintain retention and capacity similar to HPLC. Silica based particles used in columns have good mechanical strength

Further investigate and determine its identity. By utilizing chromatography, a forensic analyst can identify each material and/or chemical that were meshed together to form the sample under investigation. The identification can be determined once the sample is placed in a liquid or when absorbed on a solid surface, and the separation of the molecules becomes either mobile or stationary. This basic analytical technique is beneficial with classifying drugs that has been collected from a crime scene

In the case of the epimeric mixtures containing 2, 8, and 9, the analytes were determined using an Astec Chirobiotic T column (2.1 × 100 mm2, 5.0 μm, Advanced Separation Technologies Inc., Whippany, NJ, USA) equipped with the above-described HPLC-ESI-ion trap-MS/MS (ThermoFinnigan) instrument. This macrocyclic glycopeptide (teicoplanin)-based chiral stationary phase consists of multiple molecular interactions that contribute to distinctions in the selectivity based on the molecular shape of the analyte

Similarity that HILIC shares with normal phase HPLC is that the elution order is the same and it is in the order of increasing hydrophobicity. HILIC also requires a high amount of nonpolar mobile phase and a polar stationary phase just like normal phase HPLC. The difference between the two is that HILIC requires some water in the mobile phase to maintain a water layer on the surface so that compounds can selectively

Paper chromatography is used to separate mixtures of substances into their components. There are different types of chromatography but they are all based on the same principal. Paper chromatography is an analytical method that is used to separate colored chemicals or substances, especially pigments. They all have a stationary phase and a mobile phase. The moving substance is called the mobile phase while the stationary phase stays put. The mobile phase flows through the stationary phase and carries

including, gas-liquid chromatography, thin-layer chromatography, high-performance liquid chromatography and other related techniques such as: Electrospray Ionization-Mass Spectra, Nuclear Magnetic Resonance, UV-spectrophotometry, High pH anion exchange chromatography with pulsed amperometric detection (Table 3). 4.1 High Performance Liquid Chromatography The galacto-oligosacchrides synthesized during the reaction were analyzed by high performance liquid chromatography (HPLC) with refraction index detector

phase provides support for compound migration and is immobile on a column or plate. The mobile phase is carries the compounds, which travel from a start to an end point. Amongst all of the various types of chromatography, paper, thin layer, liquid, high pressure liquid, and gas are a few examples. This experiment utilized both paper and gas chromatography. Paper chromatography is a technique used to separate compounds using a solvent. The stationary phase is the physical paper and the solvent is

FLAVANONES FROM THE WOOD OF Morus Nigra WITH CYTOTOXIC ACTIVITY FERLINAHAYATI A,*, YANA MAOLANA SYAH B , LIA DEWI JULIAWATY B, EUIS HOLISOTAN HAKIM B a Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya Jalan Raya Palembang Prabumulih Km 32, Ogan Ilir, South Sumatera, Indonesia 30622 b Natural Product Research Group, Department of Chemistry, Institut Teknologi Bandung Jalan Ganesha 10, Bandung, Indonesia 40132 * Corresponding author, tel/fax

CHAPTER TWO RESULTS AND DISCUSSION 2.1 Synthesis of 1,3-butadienes Dienes can be synthesised from aldehydes and ketones using the Wittig reaction. The Wittig reaction facilitates the synthesis of new carbon-carbon double bonds at specific locations in aldehydes and ketones (Bernard & Ford, 1983). The overall reaction mechanism is shown in Figure 1. Protocols for synthesising 1,3-butadienes from aldehydes and ketones have been established in the literature. The synthesis protocol proposed by

Phlomis Younghusbandii Mukerjee is a perennial herb (family Labiatae) and grows wildly in Qinghai-Tibet Plateau of China. It has been widely used to treat colds, coughs, sores ulceration, rheumatoid arthritis, pneumonia, bronchitis and other diseases for thousands years. In this paper, 5 iridoid glycosides (sesamoside, shanzhiside methylester, 7, 8-dehydropenstemoside, penstemoside and 8-O-acetylshanzhiside methylester) and 3 phenylethanoid glycosides (acteoside, alyssonoside, and isoacteoside) in