High Performance Work Essay

original values.Various methods have been discussed in the literature for determination of Famotidine and Flurbiprofen tablets like spectrophotometric analysis [2], potentiometric [9], Electrochemical, Flow injection analysis FIA. Among them, High performance liquid chromatography is precise, specific, rapid and simple. There is a need of such HPLC methods for detection of two drugs in a single dosage form.The object of this study was to formulate the

Rooibos tea is also an alternative health beverage because it contains no harmful stimulants and no caffeine. The health properties are mainly due to the low tannin content and the high levels of minerals and the free-radical capturing properties of the unique flavonoids; C-glucoside dihydrocharcones asphalathin and nothofagin. In addition to the antispasmodic activity the rooibos tea also has antioxidant, anti-mutagenic and anti-aging

part of the SPARTAN 08’ program, using internal default settings for convergence criteria. Docking analysis was carried out on Peroxisome Proliferator Activated Receptor (PPAR) Gamma (PDB code: 2PRG). The chosen PPAR structure has been used in another work involving an antidiabetic drug [22]. The binding site was defined as all atoms within a radius of 6.0 Å from it co-crystallized ligand. The GOLD 5.2 program [23] was applied for docking calculations, using the ChemPLP score function. In order to take

phase (mobile phase). During the chromatographic development process, the mixture to be separated is distributed between the stationary and mobile phases. (Spangenberg Bernd et al 2011). It is mostly used because of its simplicity, relatively low cost, high sensitivity and relatively fast speed of separation. TLC also functions on the same principle as all other chromatography techniques. A compound has different affinities for the mobile and stationary phases, and this affects the speed at which it migrates

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences. Chromatography works on the basis that different molecules have different polarities. By allowing molecules to travel through a polar surface, it is not surprising that molecules will different polarities will travel a different amount. The substance, or stationary phase

Interestingly, studies from our laboratory and others have demonstrated a stereochemical dependence for dSp processing by enzymes in ODNs. The first instance of a dSp diastereomer difference was reported from our laboratory when Klenow Fragment (exo-) was allowed to insert dATP opposite the isomers for which dSp1-containing ODNs were found to be the more favorable substrate for insertion and bypass.32 This study was extended to in vivo experiments by Neeley, et al. to include pol II and pol IV

PART A - HPLC METHOD DEVELOPMENT AND QUANTITATIVE ANALYSIS Aim-To investigates the effect of changing the polarity of mobile phase on Retention time (RT) and Column capacity (k’). Results- Chromatogram for 60% methanol Tm- 1.597 Tables- For Retention time of compounds for 60% and 30% methanol. Table 1- Compound tR Xanthine 1.777 3-methylxanthine 2.500 Theobromine 2.612 Theophyline 2.798 Caffeine 3.865 Table1. shows retention time of five different compounds for 60% methanol. Table 2- Compound

RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Pioglitazone and Glimepiride bulk drug and in its pharmaceutical dosage form. Sujatha Samala,et.al., A simple and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of glimepiride in rat serum. The assay involves one step liquid-liquid extraction with methanol. Gliclazide was used as an internal standard. Chromatographic separation

Affinity Chromatography Lab report By- Pratibha Chaudhari 9/28/2017   Introduction- Affinity chromatography involves the property of bio-recognition for the separation of proteins based on the reversible interaction between the protein and the specific ligand bound to a chromatography matrix. It enables us to purify bio-molecule on the basis of its biological function and its chemical structure. The goal of the experiment was to gain hands-on experience in protein purification by

Advantages of UPLC: UPLC is more sensitive and selective with rapid resolving power and high-resolution performance. It decreases process cycle time and persuade quality of end-product with reduced run time and decreased cost of operation. Through the use of a novel column material of very small particle size, it provides quick analysis and increases sensitivity. It reduces the solvent consumption and raises sample throughput and also supports real-time analysis in step with manufacturing processes