Immunofluorescence

The process by which ∆^9–Tetrahydrocannabinol, more commonly known as THC, a major component of Cannabis sativa, has been found to possess anti-tumor properties of many cancer types. However, the use of THC is limited; particularly its usage during chemotherapy due to its psychotropic activity, the ability to affect mental activity, behavior, or perception. In addition, the exact mechanism in which THC produces this activity is not fully known. For these reasons, there has been debate about its incorporation

The Pathophysiology of Lyme borreliosis Lyme borreliosis or Lyme disease can be devastating both physically and mentally for victims. The disease is transmitted by a vector, specifically a tick, which infects their host by biting and infecting them with pathological spirochete bacteria known as Borrelia burgdorferi (Muschart & Blommaert, 2015). There are several species of Borrelia globally, and as a group, the bacteria are referred to as Borrelia burgdorferi sensu lato (Pearson, 2015). The

ntroduction Lyme disease has been the most commonly reported vector-borne illness in the United States since the Centers for Disease Control and Prevention started reporting it in 1991 [1]. It should be noted that Lyme disease does not occur everywhere in the US, and is heavily concentrated in upper Midwest and northeast United States [1]. This report has been compiled to provide recommendations for antimicrobial prophylactic treatment of Lyme disease once a patient encounters a tick bite. Lyme

INTRODUCTION: Symbiosis is the theme of this chapter as well as the theme throughout the world. Both organisms may benefit from the relationship or one organism may benefit while the other may suffer. When looking at life in the grand scheme, symbiosis is everywhere. It occurs within our own bodies as well as in many other organisms around the world. Evolutionary biologist, Toby Kiers, makes two valuable points when describing symbiosis. Kiers states that, “We need to separate important from harmonious

PATHOPHYSIOLOGY Celiac disease is an enteropathy mediated by an autoimmune response to gluten, a protein complex found in the endosperm of wheat, barley, and rye.1 The immune response is triggered by both environmental and genetic factors in individuals susceptible to the disease resulting in elevated levels of immunoglobulin A (IgA) antibodies to tissue transglutaminase (tTG), duodenal mucosal villi atrophy, and the inability of tight junctions to properly act as a barrier between the gastrointestinal

Introduction Lyme disease is a rapidly spreading infectious disease in the United States, with over 25,000 confirmed cases in 2013.5 It was first discovered in the early-1970’s in the town of Lyme, Connecticut when a group of children started to present with rheumatoid arthritis symptoms. Some of these children presented with a rash and researchers connected the symptoms to occurring during peak tick season. By the mid-1970’s, the researchers began describing the symptoms and coining the term

Toxoplasma gondii, Joan Ng Kiat Yee: BM304 revision guide 2015 The physiology, metabolism, biochemistry Toxoplsama gondii is an intracellular apicomplexan parasite that forms cyst coccidian in warm-blooded animals, primarily in the Felida – domestic cats (Shannon et al., 2015). The organism also causes a wide range of diseases in different intermediate hosts, ranging from animals, such as mammals and birds to human (Astrid et al., 2000). In human, this parasite poses serious threat by causing a

was delivered to patient fibroblasts and myoblasts. The snRNA from one corrected nucleus was expected to spread to surrounding uncorrected nuclei. Corrected cells were tested against a control of healthy human muscle biopsy tissue, using Immunofluorescence and PCR for Dystrophin. The hypothesis of neighbouring nuclei correction was tested by Quantitative PCR comparing expression of Dystrophin with a reporter gene, Green Fluorescent Protein, in cultures of corrected and uncorrected cells at relative

I hypothesize that O-GlcNAcylation of specific sites on Sec24C/D regulate COPII vesicle trafficking and mediate protein-protein interactions. The experiments proposed below will test this hypothesis by completion of my three aims: 1) Determine how O-GlcNAcylation of Sec24C/D affects COPII vesicle secretion under normal and ER stress conditions, 2) Characterize Sec24C/D O-GlcNAc mediated protein-protein interactions and their role in vesicle trafficking and, 3) Examine the Interplay between O-GlcNAcylation

This thesis aimed to develop culture expansion of undifferentiated human mesenchymal stem cells for potential use as autologous cell source for women for gynaecological disorders especially using small molecules, especially A83-01 (ALK 4/5/7 inhibitor). MSCs secret cytokines that act via paracrine effect to exert anti-inflammatory and immunomodulatory properties. Identification of MSCs in almost all postnatal tissues (bone marrow, adipose tissue, umbilical blood, menstrual blood, placenta and pre-/post-menopausal